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Quantitative assessment of visual microscopy as a tool for microplastic research: Recommendations for improving methods and reporting.
Kotar, Syd; McNeish, Rae; Murphy-Hagan, Clare; Renick, Violet; Lee, Chih-Fen T; Steele, Clare; Lusher, Amy; Moore, Charles; Minor, Elizabeth; Schroeder, Joseph; Helm, Paul; Rickabaugh, Keith; De Frond, Hannah; Gesulga, Kristine; Lao, Wenjian; Munno, Keenan; Thornton Hampton, Leah M; Weisberg, Stephen B; Wong, Charles S; Amarpuri, Gaurav; Andrews, Robert C; Barnett, Steven M; Christiansen, Silke; Cowger, Win; Crampond, Kévin; Du, Fangni; Gray, Andrew B; Hankett, Jeanne; Ho, Kay; Jaeger, Julia; Lilley, Claire; Mai, Lei; Mina, Odette; Lee, Eunah; Primpke, Sebastian; Singh, Samiksha; Skovly, Joakim; Slifko, Theresa; Sukumaran, Suja; van Bavel, Bert; Van Brocklin, Jennifer; Vollnhals, Florian; Wu, Chenxi; Rochman, Chelsea M.
Afiliación
  • Kotar S; Southern California Coastal Water Research Project Authority, 3535 Harbor Blvd, Costa Mesa, CA, 92656, USA.
  • McNeish R; Department of Biology, California State University, 9001 Stockdale Hwy, Bakersfield, CA, 93311, USA.
  • Murphy-Hagan C; Department of Environmental Sciences, University of California-Riverside, 900 University Ave, Riverside, CA, 92521, USA.
  • Renick V; Orange County Sanitation District, 10844 Ellis Ave, Fountain Valley, CA, 92708, USA.
  • Lee CT; Water Quality Laboratory, Metropolitan Water District of Southern California, La Verne, CA, 91750, USA.
  • Steele C; Environmental Science and Resource Management, California State University, Channel Islands, 1 University Drive, Camarillo, CA, 93012, USA.
  • Lusher A; Norwegian Institute for Water Research, Oslo, Norway; Department of Biological Sciences, University of Bergen, Bergen, Norway.
  • Moore C; The Moore Institute for Plastic Pollution Research, Long Beach, CA, 90803, USA.
  • Minor E; Department of Chemistry and Biochemistry and Large Lakes Observatory, University of Minnesota Duluth, 2205 East 5th St, Duluth, MN, 55812, USA.
  • Schroeder J; NatureWorks LLC, 17400 Medina Rd, Ste 800, Plymouth, MN, 55447, USA.
  • Helm P; Environmental Monitoring & Reporting Branch, Ontario Ministry of the Environment, Conservation and Parks, 125 Resources Road, Toronto, Ontario, M9P 3V6, Canada.
  • Rickabaugh K; RJ Lee Group, 350 Hochberg Road, Monroeville, PA, 15146, USA.
  • De Frond H; Department of Ecology and Evolutionary Biology, University of Toronto, 25 Willcocks St, Toronto, ON, M5S 3B2, Canada.
  • Gesulga K; Southern California Coastal Water Research Project Authority, 3535 Harbor Blvd, Costa Mesa, CA, 92656, USA.
  • Lao W; Southern California Coastal Water Research Project Authority, 3535 Harbor Blvd, Costa Mesa, CA, 92656, USA.
  • Munno K; Department of Ecology and Evolutionary Biology, University of Toronto, 25 Willcocks St, Toronto, ON, M5S 3B2, Canada.
  • Thornton Hampton LM; Southern California Coastal Water Research Project Authority, 3535 Harbor Blvd, Costa Mesa, CA, 92656, USA.
  • Weisberg SB; Southern California Coastal Water Research Project Authority, 3535 Harbor Blvd, Costa Mesa, CA, 92656, USA.
  • Wong CS; Southern California Coastal Water Research Project Authority, 3535 Harbor Blvd, Costa Mesa, CA, 92656, USA.
  • Amarpuri G; Eastman Chemical Company, 100 N. Eastman Rd, Kingsport, TN, 37660, USA.
  • Andrews RC; Department of Civil and Mineral Engineering, University of Toronto, 35 St. George St., Toronto, ON, M5S 1A4, Canada.
  • Barnett SM; Barnett Technical Services, 5050 Laguna Blvd Suite 112-620, Elk Grove, CA, 95758, USA.
  • Christiansen S; Fraunhofer Institute for Ceramics Technology and Systems (IKTS), Äußere Nürnbergerstr. 62, 91301 Forchheim, Germany; Institute for Nanotechnology and Correlative Microscopy (INAM), Äußere Nürnbergerstr. 62, 91301 Forchheim, Germany.
  • Cowger W; The Moore Institute for Plastic Pollution Research, Long Beach, CA, 90803, USA.
  • Crampond K; Institut des Sciences de la Mer de Rimouski, Université du Québec à Rimouski, Rimouski, QC, G5L 3A1, Canada.
  • Du F; State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai, 200062, China.
  • Gray AB; Department of Environmental Sciences, University of California-Riverside, 900 University Ave, Riverside, CA, 92521, USA.
  • Hankett J; BASF Corporation, 1609 Biddle Ave., Wyandotte, MI, 48192, USA.
  • Ho K; US Environmental Protection Agency, Atlantic Coastal Environmental Sciences Division, Narragansett, RI, 02882, USA.
  • Jaeger J; Eurofins Environment Testing Australia, Dandenong South, 3175, Australia.
  • Lilley C; Eurofins SF Analytical Laboratories, Inc., New Berlin, WI, 53151, USA.
  • Mai L; Center for Environmental Microplastics Studies, Guangdong Key Laboratory of Environmental Pollution and Health, School of Environment, Jinan University, Guangzhou, 511443, China.
  • Mina O; The Energy and Environmental Sustainability Laboratories, The Pennsylvania State University, 123 Land and Water Research Building, University Park, PA, 16802, USA.
  • Lee E; Horiba Instruments, Inc., Piscataway Township, NJ, 08854, USA.
  • Primpke S; Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Biologische Anstalt Helgoland, Helgoland, Germany.
  • Singh S; Department of Environmental Sciences, University of California-Riverside, 900 University Ave, Riverside, CA, 92521, USA.
  • Skovly J; Eurofins Environmental Testing Norway AS, Bergen, Norway.
  • Slifko T; Water Quality Laboratory, Metropolitan Water District of Southern California, La Verne, CA, 91750, USA.
  • Sukumaran S; Thermo Fisher Scientific, Pleasanton, CA, 94566, USA.
  • van Bavel B; Norwegian Institute for Water Research, Oslo, Norway.
  • Van Brocklin J; Department of Fisheries, Wildlife, and Conservation Sciences, Oregon State University, Corvallis, OR, 97331, USA.
  • Vollnhals F; Institute for Nanotechnology and Correlative Microscopy (INAM), Äußere Nürnbergerstr. 62, 91301 Forchheim, Germany.
  • Wu C; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
  • Rochman CM; Department of Ecology and Evolutionary Biology, University of Toronto, 25 Willcocks St, Toronto, ON, M5S 3B2, Canada. Electronic address: chelsea.rochman@utoronto.ca.
Chemosphere ; 308(Pt 3): 136449, 2022 Dec.
Article en En | MEDLINE | ID: mdl-36115477
Microscopy is often the first step in microplastic analysis and is generally followed by spectroscopy to confirm material type. The value of microscopy lies in its ability to provide count, size, color, and morphological information to inform toxicity and source apportionment. To assess the accuracy and precision of microscopy, we conducted a method evaluation study. Twenty-two laboratories from six countries were provided three blind spiked clean water samples and asked to follow a standard operating procedure. The samples contained a known number of microplastics with different morphologies (fiber, fragment, sphere), colors (clear, white, green, blue, red, and orange), polymer types (PE, PS, PVC, and PET), and sizes (ranging from roughly 3-2000 µm), and natural materials (natural hair, fibers, and shells; 100-7000 µm) that could be mistaken for microplastics (i.e., false positives). Particle recovery was poor for the smallest size fraction (3-20 µm). Average recovery (±StDev) for all reported particles >50 µm was 94.5 ± 56.3%. After quality checks, recovery for >50 µm spiked particles was 51.3 ± 21.7%. Recovery varied based on morphology and color, with poorest recovery for fibers and the largest deviations for clear and white particles. Experience mattered; less experienced laboratories tended to report higher concentration and had a higher variance among replicates. Participants identified opportunity for increased accuracy and precision through training, improved color and morphology keys, and method alterations relevant to size fractionation. The resulting data informs future work, constraining and highlighting the value of microscopy for microplastics.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Contaminantes Químicos del Agua / Microplásticos Tipo de estudio: Clinical_trials Límite: Humans Idioma: En Revista: Chemosphere Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Contaminantes Químicos del Agua / Microplásticos Tipo de estudio: Clinical_trials Límite: Humans Idioma: En Revista: Chemosphere Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido