Your browser doesn't support javascript.
loading
A first approach for the micropropagation of the medicinal halophyte Polygonum maritimum L. and phenolic profile of acclimatized plants.
Custódio, Luísa; Slusarczyk, Sylwester; Matkowski, Adam; Castañeda-Loaiza, Viana; Fernandes, Eliana; Pereira, Catarina; Rodrigues, Maria João.
Afiliación
  • Custódio L; Centre of Marine Sciences, Faculty of Sciences and Technology, University of Algarve, Faro, Portugal.
  • Slusarczyk S; Department of Pharmaceutical Biology and Botany, Wroclaw Medical University, Wroclaw, Poland.
  • Matkowski A; Department of Pharmaceutical Biology and Botany, Wroclaw Medical University, Wroclaw, Poland.
  • Castañeda-Loaiza V; Centre of Marine Sciences, Faculty of Sciences and Technology, University of Algarve, Faro, Portugal.
  • Fernandes E; Centre of Marine Sciences, Faculty of Sciences and Technology, University of Algarve, Faro, Portugal.
  • Pereira C; Centre of Marine Sciences, Faculty of Sciences and Technology, University of Algarve, Faro, Portugal.
  • Rodrigues MJ; Centre of Marine Sciences, Faculty of Sciences and Technology, University of Algarve, Faro, Portugal.
Front Plant Sci ; 13: 960306, 2022.
Article en En | MEDLINE | ID: mdl-36110351
Polygonum maritimum L. (sea knotgrass) belongs to a genus commonly used in folk medicine to treat inflammation-related disorders. In vitro pharmacological studies have confirmed these properties that were ascribed to bioactive flavonoids, such as myricetin and quercetin glycosides. Therefore, this study aimed at establishing a micropropagation procedure for sea knotgrass for obtaining standardized materials for its potential commercial cultivation. For that, a complete plant regeneration protocol was developed by improving shoot multiplication from nodal explants, rooting and acclimatization procedures, followed by the assessment of the phenolic profile of the in vitro-produced plants. The combination of 3 mg/L 6-benzylaminopurine (BA) + 0.1 mg/L indole-3-acetic acid (IAA) induced the maximum shoot formation (10.3), which was significantly increased from the first to the second cycle (18.3). The best rooting capacity was observed on shoots derived from the control medium (100%), followed by 2 mg/L kinetin (KIN) (97%) and 3 mg/L BA + 0.1 mg/L IAA (90%); however, the shoot number at the end of the rooting phase was higher on shoots derived from 3 mg/L BA + 0.1 mg/L IAA (6.16). The plant growth regulators used in the multiplication phase influenced survival in the acclimatization process, and plants derived from the control medium had the highest survival percentage (63.1%). Acetone extracts made from aerial organs of micropropagated sea knotgrass showed a predominance of the flavonoid myricetin-3-O-rhamnoside (8.135 mg/g). Overall, the halophyte sea knotgrass was successfully micropropagated showing its potential as a medicinal crop for the extraction of bioactive molecules.
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Plant Sci Año: 2022 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Plant Sci Año: 2022 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Suiza