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Cytohistologic immunohistochemical correlation of epithelial tubo-ovarian neoplasms: Can cell blocks substitute for tissue?
Lou, Si Kei; Hodgson, Anjelica; Nofech-Mozes, Sharon; Schwock, Joerg; Olkhov-Mitsel, Ekaterina; Mirkovic, Jelena; Ghorab, Zeina.
Afiliación
  • Lou SK; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada.
  • Hodgson A; Department of Anatomical Pathology, Laboratory Medicine Program, University Health Network, Toronto, ON, Canada.
  • Nofech-Mozes S; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada.
  • Schwock J; Department of Anatomical Pathology, Laboratory Medicine Program, University Health Network, Toronto, ON, Canada.
  • Olkhov-Mitsel E; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada.
  • Mirkovic J; Division of Anatomic Pathology, Department of Laboratory Medicine and Molecular Diagnostics, Sunnybrook Health Sciences Centre, Toronto, ON, Canada.
  • Ghorab Z; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada.
Cancer Cytopathol ; 131(2): 100-109, 2023 02.
Article en En | MEDLINE | ID: mdl-36103329
BACKGROUND: Cytologic specimens often represent the initial diagnostic material for tubo-ovarian neoplasms resulting from therapeutic paracentesis for patients presenting with high-volume ascites. However, subtyping and immunohistochemical (IHC) characterization, which have implications in preoperative management and downstream ancillary testing, are not routinely performed in many institutions. This study aims to perform cytohistologic correlation of commonly used IHC stains to establish their reliability in peritoneal fluids/washing specimens. METHODS: A retrospective search of the laboratory information systems was performed to identify peritoneal fluid/washing specimens involved by borderline or malignant epithelial tubo-ovarian neoplasms and concurrent/subsequent surgical resection specimens. Cell blocks and tissue were stained for PAX8, WT-1, p53, p16, Napsin-A, estrogen receptor, and progesterone receptor, and staining between cytological and surgical specimens was compared. RESULTS: A total of 56 case pairs were included, with the following final diagnoses on histological examination: 37 high-grade serous carcinomas, eight clear cell carcinomas, one endometrioid adenocarcinoma, two low-grade serous carcinomas, and eight serous borderline tumors. There was perfect cytohistologic correlation for PAX8 (Lin's concordance correlation coefficient [LINCCC] = 1.00) and WT-1 (LINCCC = 1.00), substantial/good correlation for p53 (LINCCC = 0.96), p16 (LINCCC = 0.93), napsin-A (LINCCC = 0.91) and ER (LINCCC = 0.77), and moderate correlation for PR (LINCCC = 0.54). CONCLUSIONS: Immunohistochemical correlation between peritoneal fluid and surgical resection specimens for tubo-ovarian neoplasms is high. Common subtypes of tubo-ovarian carcinomas can be reliably distinguished on fluids using IHC.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Ováricas / Carcinoma / Cistadenocarcinoma Seroso Límite: Female / Humans Idioma: En Revista: Cancer Cytopathol Año: 2023 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Ováricas / Carcinoma / Cistadenocarcinoma Seroso Límite: Female / Humans Idioma: En Revista: Cancer Cytopathol Año: 2023 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos