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Identification and determination of phenyl methyl carbamate released from adducted hemoglobin for methyl isocyanate exposure verification.
Donkor, Abigail B; Gyamfi, Obed A; White, Carl W; Nick, Heidi J; Rioux, Jacqueline S; Veress, Livia A; Logue, Brian A.
Afiliación
  • Donkor AB; Department of Chemistry and Biochemistry, South Dakota State University, Box 2202, Brookings, SD 57007, USA.
  • Gyamfi OA; Department of Chemistry and Biochemistry, South Dakota State University, Box 2202, Brookings, SD 57007, USA.
  • White CW; Department of Pediatrics-Pulmonary and Sleep Medicine Section, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
  • Nick HJ; Department of Pediatrics-Pulmonary and Sleep Medicine Section, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
  • Rioux JS; Department of Pediatrics-Pulmonary and Sleep Medicine Section, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
  • Veress LA; Department of Pediatrics-Pulmonary and Sleep Medicine Section, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
  • Logue BA; Department of Chemistry and Biochemistry, South Dakota State University, Box 2202, Brookings, SD 57007, USA. Electronic address: brian.logue@sdstate.edu.
J Chromatogr A ; 1681: 463454, 2022 Oct 11.
Article en En | MEDLINE | ID: mdl-36099696
Methyl isocyanate (MIC), an intermediate in the synthesis of carbamate pesticides, is a toxic industrial chemical that causes irritation and damage to the eyes, respiratory tract, and skin. Due to the high reactivity of MIC, it binds to proteins to form protein adducts. While these adducts can be used as biomarkers to verify exposure to MIC, methods to detect MIC adducts are cumbersome, typically involving enzymatic (pronase) or strong acid (Edman degradation) hydrolysis of hemoglobin. Hence, in this study, a simple method was developed which utilizes base hydrolysis of MIC-tyrosine adducts from isolated hemoglobin to form phenyl methyl carbamate (PMC), followed by rapid liquid-liquid extraction, and liquid chromatography tandem mass spectrometry analysis. The hydrolysis chemistry is the first report of base hydrolysis of a tyrosine-ß-C-hydroxo phenol bond in aqueous solution. The method produced excellent sensitivity (detection limit of 0.02 mg/kg), linearity (R2 = 0.998, percent residual accuracies > 96), and dynamic range (0.06‒15 mg/kg). The accuracy and precision (100 ± 9% and < 10% relative standard deviation, respectively) of the method were outstanding compared to existing techniques. The validated method was able to detect significantly elevated levels of PMC from hemoglobin isolated from MIC-exposed rats.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plaguicidas / Hemoglobinas Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: J Chromatogr A Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plaguicidas / Hemoglobinas Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: J Chromatogr A Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos