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Exosomal MiR-4261 mediates calcium overload in RBCs by downregulating the expression of ATP2B4 in multiple myeloma.
Bian, Sicheng; Zhang, Xialin; Lin, Leilei; Sun, Lili; Guo, Zhibo; Pan, Jie; Cui, Jiangxia; Yao, Hanbing; Xu, Jing; Hao, Zhuanghui; Wang, Yuzhu; Tong, Liguo; Bu, Xingpeng; Kong, Desheng; Liu, Nianjiao; Li, Yinghua.
Afiliación
  • Bian S; Key Laboratory of Cell Transplantation of National Health Commission, Heilongjiang Key Laboratory of Blood and Hematopoietic System, Harbin Medical University, Harbin, China.
  • Zhang X; Department of Hematology, The First Affiliated Hospital, Harbin Medical University, Harbin, China.
  • Lin L; Department of Hematology, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Taiyuan, China.
  • Sun L; Department of Hematology, Affiliated Yantai Yuhuangding Hospital, Qingdao University, Yantai, China.
  • Guo Z; Key Laboratory of Cell Transplantation of National Health Commission, Heilongjiang Key Laboratory of Blood and Hematopoietic System, Harbin Medical University, Harbin, China.
  • Pan J; Department of Hematology, The First Affiliated Hospital, Harbin Medical University, Harbin, China.
  • Cui J; Key Laboratory of Cell Transplantation of National Health Commission, Heilongjiang Key Laboratory of Blood and Hematopoietic System, Harbin Medical University, Harbin, China.
  • Yao H; Department of Hematology, The First Affiliated Hospital, Harbin Medical University, Harbin, China.
  • Xu J; Department of Pathology, Stanford University School of Medicine, Palo Alto, CA, United States.
  • Hao Z; Department of Hematology, Xi'an International Medical Center Hospital, Xi'an, China.
  • Wang Y; Department of Hematology, Xi'an International Medical Center Hospital, Xi'an, China.
  • Tong L; Institute of Hematology, The Second Hospital of Shanxi Medical University, Taiyuan, China.
  • Bu X; Institute of Hematology, The Second Hospital of Shanxi Medical University, Taiyuan, China.
  • Kong D; Key Laboratory of Cell Transplantation of National Health Commission, Heilongjiang Key Laboratory of Blood and Hematopoietic System, Harbin Medical University, Harbin, China.
  • Liu N; Department of Hematology, The First Affiliated Hospital, Harbin Medical University, Harbin, China.
  • Li Y; Central Laboratory, Shanxi Academy of Traditional Chinese Medicine, Taiyuan, China.
Front Oncol ; 12: 978755, 2022.
Article en En | MEDLINE | ID: mdl-36091107
Background: Hypercalcemia induced by multiple myeloma (MM) affects the biological functions of excitable and non-excitable cells. However, red blood cells (RBCs) regulatory effect on calcium in hypercalcemia is still not fully understood. Methods: A total of 113 patients with MM osteolytic lesions were studied retrospectively. Flow cytometry and atomic absorption spectroscopy were used to detect calcium content. Immunofluorescence and Western blotting were used to investigate protein expression. GEO and miRNA databases were used to screen miRNAs. Exosomal miR-4261 migration was investigated by Transwell assay. Dual-luciferase assays confirmed the targeting relationship between miR-4261 and ATP2B4. An RBC oxidative stress model was constructed, and Omega-Agatoxin IVA was used to study the role of plasma membrane Ca2+-ATPase 4 (PMCA4) in RBCs. Results: The results showed that MM RBCs had calcium overload, and serum calcium levels increased as the number of RBCs decreased. The expression of PMCA4 in MM RBCs was significantly lower than in normal RBCs. The exosomal miR-4261 produced by MM cells could be transferred to RBCs to downregulate the expression of ATP2B4. Conclusions: Studies have confirmed that RBCs experience calcium overload in MM with osteolytic lesions, which is related to the downregulation of ATP2B4 by MM exosomal miR-4261.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Oncol Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Oncol Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza