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Mouse Models for Cytomegalovirus Infections in Newborns and Adults.
Brizic, Ilija; Lisnic, Berislav; Krstanovic, Fran; Brune, Wolfram; Hengel, Hartmut; Jonjic, Stipan.
Afiliación
  • Brizic I; Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia.
  • Lisnic B; Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia.
  • Krstanovic F; Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia.
  • Brune W; Leibniz Institute for Virology, Hamburg, Germany.
  • Hengel H; Institute of Virology, Medical Center-University of Freiburg, and Faculty of Medicine, University of Freiburg, Freiburg, Germany.
  • Jonjic S; Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia.
Curr Protoc ; 2(9): e537, 2022 Sep.
Article en En | MEDLINE | ID: mdl-36083111
This article describes procedures for infecting adult mice with murine cytomegalovirus (MCMV) and for infecting newborn mice to model congenital CMV infection. Methods are included for propagating MCMV in cell cultures and preparing a more virulent form of MCMV from the salivary glands of infected mice. A plaque assay is provided for determining MCMV titers of infected tissues or virus stocks. Also, methods are described for preparing the murine embryonic fibroblasts used for propagating MCMV, and for the plaque assay. © 2022 Wiley Periodicals LLC.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Muromegalovirus / Infecciones por Citomegalovirus Límite: Animals Idioma: En Revista: Curr Protoc Año: 2022 Tipo del documento: Article País de afiliación: Croacia Pais de publicación: Estados Unidos
Texto completo
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PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Muromegalovirus / Infecciones por Citomegalovirus Límite: Animals Idioma: En Revista: Curr Protoc Año: 2022 Tipo del documento: Article País de afiliación: Croacia Pais de publicación: Estados Unidos