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A Novel Pathway of Chlorimuron-Ethyl Biodegradation by Chenggangzhangella methanolivorans Strain CHL1 and Its Molecular Mechanisms.
Yu, Zhixiong; Dai, Yumeng; Li, Tingting; Gu, Wu; Yang, Yi; Li, Xiang; Peng, Pai; Yang, Lijie; Li, Xinyu; Wang, Jian; Su, Zhencheng; Li, Xu; Xu, Mingkai; Zhang, Huiwen.
Afiliación
  • Yu Z; Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.
  • Dai Y; Basic Medical College, Shenyang Medical College, Shenyang 100034, China.
  • Li T; Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.
  • Gu W; University of Chinese Academy of Sciences, Beijing 100049, China.
  • Yang Y; Shenyang Research Institute of Chemical Industry, Shenyang 110021, China.
  • Li X; Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.
  • Peng P; University of Chinese Academy of Sciences, Beijing 100049, China.
  • Yang L; Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.
  • Li X; Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.
  • Wang J; Shenyang Research Institute of Chemical Industry, Shenyang 110021, China.
  • Su Z; Shenyang Research Institute of Chemical Industry, Shenyang 110021, China.
  • Li X; Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.
  • Xu M; Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.
  • Zhang H; Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.
Int J Mol Sci ; 23(17)2022 Aug 31.
Article en En | MEDLINE | ID: mdl-36077288
Chlorimuron-ethyl is a widely used herbicide in agriculture. However, uncontrolled chlorimuron-ethyl application causes serious environmental problems. Chlorimuron-ethyl can be effectively degraded by microbes, but the underlying molecular mechanisms are not fully understood. In this study, we identified the possible pathways and key genes involved in chlorimuron-ethyl degradation by the Chenggangzhangella methanolivorans strain CHL1, a Methylocystaceae strain with the ability to degrade sulfonylurea herbicides. Using a metabolomics method, eight intermediate degradation products were identified, and three pathways, including a novel pyrimidine-ring-opening pathway, were found to be involved in chlorimuron-ethyl degradation by strain CHL1. Transcriptome sequencing indicated that three genes (atzF, atzD, and cysJ) are involved in chlorimuron-ethyl degradation by strain CHL1. The gene knock-out and complementation techniques allowed for the functions of the three genes to be identified, and the enzymes involved in the different steps of chlorimuron-ethyl degradation pathways were preliminary predicted. The results reveal a previously unreported pathway and the key genes of chlorimuron-ethyl degradation by strain CHL1, which have implications for attempts to enrich the biodegradation mechanism of sulfonylurea herbicides and to construct engineered bacteria in order to remove sulfonylurea herbicide residues from environmental media.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Contaminantes del Suelo / Methylocystaceae / Herbicidas Idioma: En Revista: Int J Mol Sci Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Contaminantes del Suelo / Methylocystaceae / Herbicidas Idioma: En Revista: Int J Mol Sci Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza