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Generation of two induced pluripotent stem cell lines (RCMGi005-A/B) from human skin fibroblasts of a cystic fibrosis patient with homozygous F508del mutation in CFTR gene.
Panchuk, Irina; Kondrateva, Ekaterina; Demchenko, Anna; Grigorieva, Olga; Erofeeva, Anastasija; Amelina, Elena; Tabakov, Vyacheslav; Orlova, Maria; Voronina, Ekaterina; Pozhitnova, Victoria; Lavrov, Alexander; Smirnikhina, Svetlana; Kutsev, Sergey.
Afiliación
  • Panchuk I; Research Centre for Medical Genetics, Moscow, Russian Federation. Electronic address: irina.panchuk42@gmail.com.
  • Kondrateva E; Research Centre for Medical Genetics, Moscow, Russian Federation.
  • Demchenko A; Research Centre for Medical Genetics, Moscow, Russian Federation.
  • Grigorieva O; Research Centre for Medical Genetics, Moscow, Russian Federation.
  • Erofeeva A; Research Centre for Medical Genetics, Moscow, Russian Federation.
  • Amelina E; The Research Institute of Pulmonology, Moscow 115682, Russian Federation.
  • Tabakov V; Research Centre for Medical Genetics, Moscow, Russian Federation.
  • Orlova M; Research Centre for Medical Genetics, Moscow, Russian Federation.
  • Voronina E; Research Centre for Medical Genetics, Moscow, Russian Federation.
  • Pozhitnova V; Research Centre for Medical Genetics, Moscow, Russian Federation.
  • Lavrov A; Research Centre for Medical Genetics, Moscow, Russian Federation.
  • Smirnikhina S; Research Centre for Medical Genetics, Moscow, Russian Federation.
  • Kutsev S; Research Centre for Medical Genetics, Moscow, Russian Federation.
Stem Cell Res ; 64: 102896, 2022 10.
Article en En | MEDLINE | ID: mdl-36067639
Induced pluripotent stem cells (iPSCs) was successfully generated from skin fibroblast obtained from patient with cystic fibrosis by using non-integrating, viral CytoTune™-iPS 2.0 Sendai Reprogramming Kit, which contain three vectors preparation: polycistronic Klf4-Oct3/4-Sox2, cMyc, and Klf4. Created iPSC lines showed a normal karyotype, expressed pluripotency markers and demonstrated the potential to differentiate into three germ layers in spontaneous differentiation assay.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fibrosis Quística / Células Madre Pluripotentes Inducidas Límite: Humans Idioma: En Revista: Stem Cell Res Año: 2022 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fibrosis Quística / Células Madre Pluripotentes Inducidas Límite: Humans Idioma: En Revista: Stem Cell Res Año: 2022 Tipo del documento: Article Pais de publicación: Reino Unido