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Delivery of miRNAs through Metal-Organic Framework Nanoparticles for Assisting Neural Stem Cell Therapy for Ischemic Stroke.
Yang, Hongru; Han, Min; Li, Jian; Ke, Hongfei; Kong, Ying; Wang, Wenhan; Wang, Liang; Ma, Wenjun; Qiu, Jichuan; Wang, Xiwei; Xin, Tao; Liu, Hong.
Afiliación
  • Yang H; State Key Laboratory of Crystal Materials, Shandong University, Jinan, Shandong 250100, People's Republic of China.
  • Han M; Department of Neurosurgery, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, Shandong 250014, People's Republic of China.
  • Li J; Medical Science and Technology Innovation Center, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250117, People's Republic of China.
  • Ke H; State Key Laboratory of Separation Membranes and Membrane Processes, Tiangong University, Tianjin 300387, People's Republic of China.
  • Kong Y; Department of Physiology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, People's Republic of China.
  • Wang W; State Key Laboratory of Crystal Materials, Shandong University, Jinan, Shandong 250100, People's Republic of China.
  • Wang L; State Key Laboratory of Crystal Materials, Shandong University, Jinan, Shandong 250100, People's Republic of China.
  • Ma W; State Key Laboratory of Crystal Materials, Shandong University, Jinan, Shandong 250100, People's Republic of China.
  • Qiu J; State Key Laboratory of Crystal Materials, Shandong University, Jinan, Shandong 250100, People's Republic of China.
  • Wang X; State Key Laboratory of Crystal Materials, Shandong University, Jinan, Shandong 250100, People's Republic of China.
  • Xin T; Institute of Novel Semiconductors, Shandong University, Jinan, Shandong 250100, People's Republic of China.
  • Liu H; Department of Neurosurgery, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, Shandong 250014, People's Republic of China.
ACS Nano ; 16(9): 14503-14516, 2022 09 27.
Article en En | MEDLINE | ID: mdl-36065995
Stroke is the most common cause of disability globally. Neural stem cell (NSC) therapy, which can replace lost and damaged neurons, has been proposed as a potential treatment for stroke. The therapeutic efficacy of NSC therapy is hindered by the fact that only a small number of NSCs undergo neuronal differentiation. Neuron-specific miR-124, which promotes the differentiation of NSCs into mature neurons, can be combined with NSC therapy to cure ischemic stroke. However, the instability and poor internalization of miR-124 seriously hamper its broad clinical application. Herein, an innovative strategy involving delivery of miR-124 via a Ca-MOF@miR-124 nanodelivery system, which effectively prevents the degradation of miR-124 by nucleases and promotes the internalization of miR-124 by NSCs, is presented. The effect of accelerated neuronal directed differentiation of NSCs was assessed through in vitro cell experiments, and the clinical application potential of this nanodelivery system for the treatment of ischemic stroke was assessed through in vivo experiments involving the combination of NSC therapy and Ca-MOF@miR-124 nanoparticles. The results indicate that Ca-MOF@miR-124 nanoparticles can promote the differentiation of NSCs into mature neurons with electrophysiological function within 5 days. The differentiation rate of cells treated with Ca-MOF@miR-124 nanoparticles was at least 5 days faster than that of untreated cells. Moreover, Ca-MOF@miR-124 nanoparticles decreased the ischemic area to almost normal levels by day 7. The combination of Ca-MOF@miR-124 nanoparticles and NSC therapy will enhance the treatment of traumatic nerve injury and neurodegenerative diseases.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Accidente Cerebrovascular / MicroARNs / Nanopartículas / Células-Madre Neurales / Estructuras Metalorgánicas / Accidente Cerebrovascular Isquémico Límite: Humans Idioma: En Revista: ACS Nano Año: 2022 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Accidente Cerebrovascular / MicroARNs / Nanopartículas / Células-Madre Neurales / Estructuras Metalorgánicas / Accidente Cerebrovascular Isquémico Límite: Humans Idioma: En Revista: ACS Nano Año: 2022 Tipo del documento: Article Pais de publicación: Estados Unidos