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Unfolding of the SARS-CoV-2 spike protein through infrared and ultraviolet-C radiation based disinfection.
Mahanta, Nilkamal; Sharma, Swati; Sharma, Laipubam Gayatri; Pandey, Lalit M; Dixit, Uday Shanker.
Afiliación
  • Mahanta N; Department of Mechanical Engineering, Indian Institute of Technology Guwahati, India.
  • Sharma S; Bio-Interface and Environmental Engineering Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, India.
  • Sharma LG; Bio-Interface and Environmental Engineering Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, India.
  • Pandey LM; Bio-Interface and Environmental Engineering Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, India.
  • Dixit US; Department of Mechanical Engineering, Indian Institute of Technology Guwahati, India. Electronic address: uday@iitg.ac.in.
Int J Biol Macromol ; 221: 71-82, 2022 Nov 30.
Article en En | MEDLINE | ID: mdl-36063893
The spreading of coronavirus from contacting surfaces and aerosols created a pandemic around the world. To prevent the transmission of SARS-CoV-2 virus and other contagious microbes, disinfection of contacting surfaces is necessary. In this study, a disinfection box equipped with infrared (IR) radiation heating and ultraviolet-C (UV-C) radiation is designed and tested for its disinfection ability against pathogenic bacteria and SARS-CoV-2 spike protein. The killing of a Gram-positive, namely, S. aureus and a Gram-negative namely, S. typhi bacteria was studied followed by the inactivation of the spike protein. The experimental parameters were optimized using a statistical tool. For the broad-spectrum antibacterial activity, the optimum condition was holding at 65.61 °C for 13.54 min. The killing of the bacterial pathogen occurred via rupturing the cell walls as depicted by electron microscopy. Further, the unfolding of SARS-CoV-2 spike protein and RNase A was studied under IR and UV-C irradiations at the aforesaid optimized condition. The unfolding of both the proteins was confirmed by changes in the secondary structure, particularly an increase in ß-sheets and a decrease in α-helixes. Remarkably, the higher penetration depth of IR waves up to subcutaneous tissue resulted in lower optimum disinfection temperature, <70 °C in vogue. Thus, the combined UV-C and IR radiation is effective in killing the pathogenic bacteria and denaturing the glycoproteins.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glicoproteína de la Espiga del Coronavirus / COVID-19 Límite: Humans Idioma: En Revista: Int J Biol Macromol Año: 2022 Tipo del documento: Article País de afiliación: India Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glicoproteína de la Espiga del Coronavirus / COVID-19 Límite: Humans Idioma: En Revista: Int J Biol Macromol Año: 2022 Tipo del documento: Article País de afiliación: India Pais de publicación: Países Bajos