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Cyclic ADP ribose isomers: Production, chemical structures, and immune signaling.
Manik, Mohammad K; Shi, Yun; Li, Sulin; Zaydman, Mark A; Damaraju, Neha; Eastman, Samuel; Smith, Thomas G; Gu, Weixi; Masic, Veronika; Mosaiab, Tamim; Weagley, James S; Hancock, Steven J; Vasquez, Eduardo; Hartley-Tassell, Lauren; Kargios, Nestoras; Maruta, Natsumi; Lim, Bryan Y J; Burdett, Hayden; Landsberg, Michael J; Schembri, Mark A; Prokes, Ivan; Song, Lijiang; Grant, Murray; DiAntonio, Aaron; Nanson, Jeffrey D; Guo, Ming; Milbrandt, Jeffrey; Ve, Thomas; Kobe, Bostjan.
Afiliación
  • Manik MK; School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia.
  • Shi Y; Australian Infectious Diseases Research Centre, The University of Queensland, Brisbane, QLD 4072, Australia.
  • Li S; Institute for Glycomics, Griffith University, Southport, QLD 4222, Australia.
  • Zaydman MA; School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia.
  • Damaraju N; Australian Infectious Diseases Research Centre, The University of Queensland, Brisbane, QLD 4072, Australia.
  • Eastman S; Department of Pathology and Immunology, Washington University School of Medicine in St. Louis, St. Louis, MO 63100, USA.
  • Smith TG; Department of Developmental Biology, Washington University School of Medicine in St. Louis, St. Louis, MO 63100, USA.
  • Gu W; Department of Genetics, Washington University School of Medicine in St. Louis, St. Louis, MO 63100, USA.
  • Masic V; Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, NE 68583, USA.
  • Mosaiab T; Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
  • Weagley JS; School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia.
  • Hancock SJ; Australian Infectious Diseases Research Centre, The University of Queensland, Brisbane, QLD 4072, Australia.
  • Vasquez E; Institute for Glycomics, Griffith University, Southport, QLD 4222, Australia.
  • Hartley-Tassell L; Institute for Glycomics, Griffith University, Southport, QLD 4222, Australia.
  • Kargios N; Edison Family Center for Genome Sciences and Systems Biology, Washington University School of Medicine in St. Louis, St. Louis, MO 63110, USA.
  • Maruta N; School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia.
  • Lim BYJ; Australian Infectious Diseases Research Centre, The University of Queensland, Brisbane, QLD 4072, Australia.
  • Burdett H; Institute for Glycomics, Griffith University, Southport, QLD 4222, Australia.
  • Landsberg MJ; Institute for Glycomics, Griffith University, Southport, QLD 4222, Australia.
  • Schembri MA; School of Life Sciences, University of Warwick, Coventry CV4 7AL, UK.
  • Prokes I; School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia.
  • Song L; Australian Infectious Diseases Research Centre, The University of Queensland, Brisbane, QLD 4072, Australia.
  • Grant M; School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia.
  • DiAntonio A; Australian Infectious Diseases Research Centre, The University of Queensland, Brisbane, QLD 4072, Australia.
  • Nanson JD; School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia.
  • Guo M; Australian Infectious Diseases Research Centre, The University of Queensland, Brisbane, QLD 4072, Australia.
  • Milbrandt J; School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia.
  • Ve T; Australian Infectious Diseases Research Centre, The University of Queensland, Brisbane, QLD 4072, Australia.
  • Kobe B; School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia.
Science ; 377(6614): eadc8969, 2022 09 30.
Article en En | MEDLINE | ID: mdl-36048923
Cyclic adenosine diphosphate (ADP)-ribose (cADPR) isomers are signaling molecules produced by bacterial and plant Toll/interleukin-1 receptor (TIR) domains via nicotinamide adenine dinucleotide (oxidized form) (NAD+) hydrolysis. We show that v-cADPR (2'cADPR) and v2-cADPR (3'cADPR) isomers are cyclized by O-glycosidic bond formation between the ribose moieties in ADPR. Structures of 2'cADPR-producing TIR domains reveal conformational changes that lead to an active assembly that resembles those of Toll-like receptor adaptor TIR domains. Mutagenesis reveals a conserved tryptophan that is essential for cyclization. We show that 3'cADPR is an activator of ThsA effector proteins from the bacterial antiphage defense system termed Thoeris and a suppressor of plant immunity when produced by the effector HopAM1. Collectively, our results reveal the molecular basis of cADPR isomer production and establish 3'cADPR in bacteria as an antiviral and plant immunity-suppressing signaling molecule.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacterias / Proteínas Bacterianas / ADP-Ribosil Ciclasa / ADP-Ribosa Cíclica / Proteínas Adaptadoras del Transporte Vesicular / Receptores Toll-Like / Inmunidad de la Planta Idioma: En Revista: Science Año: 2022 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacterias / Proteínas Bacterianas / ADP-Ribosil Ciclasa / ADP-Ribosa Cíclica / Proteínas Adaptadoras del Transporte Vesicular / Receptores Toll-Like / Inmunidad de la Planta Idioma: En Revista: Science Año: 2022 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos