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Comparative Genomic and Phylogenetic Analysis of Forty Gentiana Chloroplast Genomes.
Deng, Gang; Gao, Ran-Ran; Wang, Wen-Ting; Wu, Tian-Ze; Zhang, Yong-Ping; Wang, Bo; Xiang, Li; Liu, Xia.
Afiliación
  • Deng G; School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, 430070 Wuhan, Hubei, China.
  • Gao RR; Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, 100700 Beijing, China.
  • Wang WT; Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, 100700 Beijing, China.
  • Wu TZ; School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, 430070 Wuhan, Hubei, China.
  • Zhang YP; National Engineering Technology Research Center for Miao Medicine, College of Pharmaceutical Sciences, Guizhou University of Traditional Chinese Medicine, 550025 Guiyang, Guizhou, China.
  • Wang B; National Engineering Technology Research Center for Miao Medicine, College of Pharmaceutical Sciences, Guizhou University of Traditional Chinese Medicine, 550025 Guiyang, Guizhou, China.
  • Xiang L; Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, 100700 Beijing, China.
  • Liu X; School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, 430070 Wuhan, Hubei, China.
Front Biosci (Landmark Ed) ; 27(8): 236, 2022 08 09.
Article en En | MEDLINE | ID: mdl-36042168
BACKGROUND: Gentiana plants, which have great medicinal and ornamental value, are widely distributed in diverse habitats and have complex taxonomy. Here 40 Gentiana chloroplast genomes were used for comparative genomic analysis and divergence time estimation. METHODS: The complete chloroplast genome of G. rhodantha was sequenced, assembled, and annotated. Comparative genomic and phylogenetic analysis were provided for variation analysis of Gentiana. RESULTS: Gentiana species satisfy the characteristics of intra-Sect conservation and inter-Sect variation in chloroplast genome structure and IR boundaries. All Gentiana Sects can be clustered into a single one and separated from each other; however, Ser. Apteroideae and Ser. Confertifoliae in Sect. Monopodiae are more closely related to Sect. Frigida and Sect. Cruciata, respectively. Gentiana has experienced two large gene loss events; the first, the collective loss of the rps16 gene at genus formation and the second, the collective loss of the ndh gene when Ser. Ornatae and Ser. Verticillatae completed their differentiation. Comparative genomic analysis support that Sect. Stenogyne and Sect. Otophora became the independent genera Metagentiana and Kuepferia. Seven divergence hotspot regions were screened based on Pi values, and could serve as DNA-specific barcodes for Gentiana. CONCLUSIONS: This study provides a further theoretical basis for taxonomic analysis, genetic diversity, evolutionary mechanism and molecular identification in Gentiana.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Gentiana / Genoma del Cloroplasto Tipo de estudio: Prognostic_studies Idioma: En Revista: Front Biosci (Landmark Ed) Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Singapur

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Gentiana / Genoma del Cloroplasto Tipo de estudio: Prognostic_studies Idioma: En Revista: Front Biosci (Landmark Ed) Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Singapur