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Detection of Equus Caballus Papillomavirus Type-2 in Asymptomatic Italian Horses.
Cappelli, Katia; Ciucis, Chiara Grazia De; Mecocci, Samanta; Nervo, Tiziana; Crescio, Maria Ines; Pepe, Marco; Gialletti, Rodolfo; Pietrucci, Daniele; Migone, Laura Federica; Turco, Silvia; Mechelli, Luca; Passamonti, Fabrizio; Drago, Carlo; Donato, Gian Guido; Varello, Katia; Modesto, Paola; Chillemi, Giovanni; Ghelardi, Alessandro; Razzuoli, Elisabetta.
Afiliación
  • Cappelli K; Dipartimento di Medicina Veterinaria, 06126 Perugia, Italy.
  • Ciucis CG; Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, National Reference Center of Veterinary and comparative Oncology (CEROVEC), 16129 Genova, Italy.
  • Mecocci S; Dipartimento di Medicina Veterinaria, 06126 Perugia, Italy.
  • Nervo T; Dipartimento di Scienze Veterinarie, 10095 Grugliasco, Italy.
  • Crescio MI; Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, National Reference Center of Veterinary and comparative Oncology (CEROVEC), 16129 Genova, Italy.
  • Pepe M; Dipartimento di Medicina Veterinaria, 06126 Perugia, Italy.
  • Gialletti R; Dipartimento di Medicina Veterinaria, 06126 Perugia, Italy.
  • Pietrucci D; Dipartimento per la Innovazione nei sistemi biologici, agroalimentari e forestali (DIBAF), Università degli Studi della Tuscia, 01100 Viterbo, Italy.
  • Migone LF; Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, National Reference Center of Veterinary and comparative Oncology (CEROVEC), 16129 Genova, Italy.
  • Turco S; Dipartimento di Scienze Agrarie e Forestali (DAFNE), Università degli Studi della Tuscia, 01100 Viterbo, Italy.
  • Mechelli L; Dipartimento di Medicina Veterinaria, 06126 Perugia, Italy.
  • Passamonti F; Dipartimento di Medicina Veterinaria, 06126 Perugia, Italy.
  • Drago C; Dipartimento di Statistica Economica, Università degli Studi Niccolò Cusano, 00166 Roma, Italy.
  • Donato GG; Dipartimento di Scienze Veterinarie, 10095 Grugliasco, Italy.
  • Varello K; Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, National Reference Center of Veterinary and comparative Oncology (CEROVEC), 16129 Genova, Italy.
  • Modesto P; Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, National Reference Center of Veterinary and comparative Oncology (CEROVEC), 16129 Genova, Italy.
  • Chillemi G; Dipartimento per la Innovazione nei sistemi biologici, agroalimentari e forestali (DIBAF), Università degli Studi della Tuscia, 01100 Viterbo, Italy.
  • Ghelardi A; Azienda USL Toscana Nord-Ovest UOC Ostetricia e Ginecologia, Nuovo Ospedale Apuane, 54100 Massa, Italy.
  • Razzuoli E; Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, National Reference Center of Veterinary and comparative Oncology (CEROVEC), 16129 Genova, Italy.
Viruses ; 14(8)2022 07 31.
Article en En | MEDLINE | ID: mdl-36016317
Equine Papillomavirus 2 (EcPV2) is responsible for squamous cell carcinomas (eSCCs) of external genitalia of both male and female horses. However, few studies report the EcPV2 prevalence among healthy horses. Currently, the lack of these data does not permit identifying at-risk populations and, thus, developing screening protocols aimed at the early detection of the infection, as for humans. The aim of our study was to estimate the genoprevalence of EcPV2 in clinically healthy horses in Italy and to evaluate their innate immune response. For this purpose, penile and vulvar swabs of 234 healthy horses were collected through sampling with sterile cytobrushes. Nucleic acids were isolated and EcPV2-L1 presence (DNA) and gene expression (RNA) were checked by RT-qPCR. Our results showed EcPV2-L1 DNA presence in 30.3% of the samples and L1 expression in 48% of the positive samples. No statistically significant differences were found in genoprevalence in relation to sex, age, and origin, while, concerning breeds, the Thoroughbred had the highest risk of infection. Concerning specifically the mares, 40.2% of them resulted in being positive for EcPV2; our findings show a major positivity in pluriparous (p = 0.0111) and mares subjected to natural reproduction (p = 0.0037). Moreover, samples expressing L1 showed an increased expression of IL1B (p = 0.0139) and IL12p40 (p = 0.0133) and a decreased expression of RANKL (p = 0.0229) and TGFB (p = 0.0177). This finding suggests the presence of an effective immune response, which could explain the low incidence of SCCs in positive horses, despite a high EcPV2 genoprevalence (30%).
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carcinoma de Células Escamosas / Infecciones por Papillomavirus / Enfermedades de los Caballos Tipo de estudio: Diagnostic_studies / Guideline / Prognostic_studies / Risk_factors_studies / Screening_studies Límite: Animals / Female / Humans / Male Idioma: En Revista: Viruses Año: 2022 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza
Texto completo
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carcinoma de Células Escamosas / Infecciones por Papillomavirus / Enfermedades de los Caballos Tipo de estudio: Diagnostic_studies / Guideline / Prognostic_studies / Risk_factors_studies / Screening_studies Límite: Animals / Female / Humans / Male Idioma: En Revista: Viruses Año: 2022 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza