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Distinct Uptake Routes Participate in Silver Nanoparticle Engulfment by Earthworm and Human Immune Cells.
Kokhanyuk, Bohdana; Vántus, Viola Bagóné; Radnai, Balázs; Vámos, Eszter; Kajner, Gyula; Galbács, Gábor; Telek, Elek; Mészáros, Mária; Deli, Mária A; Németh, Péter; Engelmann, Péter.
Afiliación
  • Kokhanyuk B; Department of Immunology and Biotechnology, Clinical Center, Medical School, University of Pécs, H-7624 Pécs, Hungary.
  • Vántus VB; Department of Biochemistry and Medicinal Chemistry, Medical School, University of Pécs, H-7624 Pécs, Hungary.
  • Radnai B; Department of Biochemistry and Medicinal Chemistry, Medical School, University of Pécs, H-7624 Pécs, Hungary.
  • Vámos E; Department of Biochemistry and Medicinal Chemistry, Medical School, University of Pécs, H-7624 Pécs, Hungary.
  • Kajner G; Department of Inorganic and Analytical Chemistry, Faculty of Science and Informatics, University of Szeged, H-6720 Szeged, Hungary.
  • Galbács G; Department of Inorganic and Analytical Chemistry, Faculty of Science and Informatics, University of Szeged, H-6720 Szeged, Hungary.
  • Telek E; Department of Biophysics, Medical School, University of Pécs, H-7624 Pécs, Hungary.
  • Mészáros M; Institute of Biophysics, Biological Research Centre, Eötvös Loránd Research Network, H-6726 Szeged, Hungary.
  • Deli MA; Institute of Biophysics, Biological Research Centre, Eötvös Loránd Research Network, H-6726 Szeged, Hungary.
  • Németh P; Department of Immunology and Biotechnology, Clinical Center, Medical School, University of Pécs, H-7624 Pécs, Hungary.
  • Engelmann P; Department of Immunology and Biotechnology, Clinical Center, Medical School, University of Pécs, H-7624 Pécs, Hungary.
Nanomaterials (Basel) ; 12(16)2022 Aug 17.
Article en En | MEDLINE | ID: mdl-36014683
The consequences of engineered silver nanoparticle (AgNP) exposure and cellular interaction with the immune system are poorly understood. The immunocytes of the Eisenia andrei earthworm are frequently applied in ecotoxicological studies and possess functional similarity to vertebrate macrophages. Hence, we characterized and compared the endocytosis mechanisms for the uptake of 75 nm AgNPs by earthworm coelomocytes, human THP-1 monocytes, and differentiated THP-1 (macrophage-like) cells. Our results indicate that microtubule-dependent, scavenger-receptor, and PI3K signaling-mediated macropinocytosis are utilized during AgNP engulfment by human THP-1 and differentiated THP-1 cells. However, earthworm coelomocytes employ actin-dependent phagocytosis during AgNPs uptake. In both human and earthworm immunocytes, AgNPs were located in the cytoplasm, within the endo-/lysosomes. We detected that the internalization of AgNPs is TLR/MyD88-dependent, also involving the bactericidal/permeability-increasing protein (BPI) in the case of human immunocytes. The exposure led to decreased mitochondrial respiration in human immunocytes; however, in coelomocytes, it enhanced respiratory parameters. Our findings provide more data about NP trafficking as nano-carriers in the nanomedicine field, as well as contribute to an understanding of the ecotoxicological consequences of nanoparticle exposure.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Nanomaterials (Basel) Año: 2022 Tipo del documento: Article País de afiliación: Hungria Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Nanomaterials (Basel) Año: 2022 Tipo del documento: Article País de afiliación: Hungria Pais de publicación: Suiza