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Human gut bifidobacteria inhibit the growth of the opportunistic fungal pathogen Candida albicans.
Ricci, Liviana; Mackie, Joanna; Donachie, Gillian E; Chapuis, Ambre; Mezerová, Kristýna; Lenardon, Megan D; Brown, Alistair J P; Duncan, Sylvia H; Walker, Alan W.
Afiliación
  • Ricci L; Rowett Institute, University of Aberdeen, Aberdeen, AB25 2ZD, United Kingdom.
  • Mackie J; CIBIO - Department of Cellular, Computational and Integrative Biology, University of Trento, Trento, 38123, Italy.
  • Donachie GE; Aberdeen Fungal Group, Institute of Medical Sciences, University of Aberdeen, Aberdeen, AB25 2ZD, United Kingdom.
  • Chapuis A; Rowett Institute, University of Aberdeen, Aberdeen, AB25 2ZD, United Kingdom.
  • Mezerová K; Aberdeen Fungal Group, Institute of Medical Sciences, University of Aberdeen, Aberdeen, AB25 2ZD, United Kingdom.
  • Lenardon MD; Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University Olomouc, Olomouc, 77515, Czech Republic.
  • Brown AJP; Aberdeen Fungal Group, Institute of Medical Sciences, University of Aberdeen, Aberdeen, AB25 2ZD, United Kingdom.
  • Duncan SH; School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
  • Walker AW; Aberdeen Fungal Group, Institute of Medical Sciences, University of Aberdeen, Aberdeen, AB25 2ZD, United Kingdom.
FEMS Microbiol Ecol ; 98(10)2022 09 19.
Article en En | MEDLINE | ID: mdl-36007932
The human gut microbiota protects the host from invading pathogens and the overgrowth of indigenous opportunistic species via a process called colonization resistance. Here, we investigated the antagonistic activity of human gut bacteria towards Candida albicans, an opportunistic fungal pathogen that can cause severe infections in susceptible individuals. Coculture batch incubations of C. albicans in the presence of faecal microbiota from six healthy individuals revealed varying levels of inhibitory activity against C. albicans. 16S rRNA gene amplicon profiling of these faecal coculture bacterial communities showed that the Bifidobacteriaceae family, and Bifidobacterium adolescentis in particular, were most correlated with antagonistic activity against C. albicans. Follow-up mechanistic studies performed under anaerobic conditions confirmed that culture supernatants of Bifidobacterium species, particularly B. adolescentis, inhibited C. albicans in vitro. Fermentation acids (FA), including acetate and lactate, present in the bifidobacterial supernatants were important contributors to inhibitory activity. However, increasing the pH of both bacterial supernatants and mixtures of FA reduced their anti-Candida effects, indicating a combinatorial effect of prevailing pH and FA. This work, therefore, demonstrates potential mechanisms underpinning gut microbiome-mediated colonization resistance against C. albicans, and identifies particularly inhibitory components such as bifidobacteria and FA as targets for further study.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Candida albicans / Microbioma Gastrointestinal Límite: Humans Idioma: En Revista: FEMS Microbiol Ecol Año: 2022 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Candida albicans / Microbioma Gastrointestinal Límite: Humans Idioma: En Revista: FEMS Microbiol Ecol Año: 2022 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Reino Unido