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Adjustable biodegradability of low-swelling hydrogels prepared from recombinant peptides based on human collagen type 1.
Nakano, Takashi; Yamanaka, Hiroki; Sakamoto, Michiharu; Aiki, Yasuhiro; Yanase, Naoto; Hori, Ritsuko; Katayama, Yasuhiro; Tsuge, Itaru; Saito, Susumu; Morimoto, Naoki.
Afiliación
  • Nakano T; Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, 38049Kyoto University, Kyoto, Japan.
  • Yamanaka H; Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, 38049Kyoto University, Kyoto, Japan.
  • Sakamoto M; Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, 38049Kyoto University, Kyoto, Japan.
  • Aiki Y; Bio Science & Engineering Laboratory, 34778FUJIFILM Corporation, Kanagawa, Japan.
  • Yanase N; Analysis Technology Center, 612992FUJIFILM Corporation, Kanagawa, Japan.
  • Hori R; Analysis Technology Center, 612992FUJIFILM Corporation, Kanagawa, Japan.
  • Katayama Y; Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, 38049Kyoto University, Kyoto, Japan.
  • Tsuge I; Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, 38049Kyoto University, Kyoto, Japan.
  • Saito S; Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, 38049Kyoto University, Kyoto, Japan.
  • Morimoto N; Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, 38049Kyoto University, Kyoto, Japan.
J Biomater Appl ; 37(5): 881-890, 2022 11.
Article en En | MEDLINE | ID: mdl-36007126
An ideal hydrogel for tissue engineering and regenerative therapy is cytocompatible, biocompatible, and has low-swelling characteristics. Recently, a novel low-swelling hydrogel with a homogenous structure was developed by crosslinking a recombinant peptide, modeled on human collagen type 1 (RCPhC1), with a four-arm polyethylene glycol (tetra-PEG). Here, we hypothesized that the biodegradability of the RCPhC1 hydrogel was adjustable by altering its initial polymer concentration. Three types of RCPhC1 hydrogels were prepared using the initial polymer at different concentrations, and their morphology, swelling ratio, collagenase degradability, cytocompatibility, biocompatibility, and biodegradability were compared. The results revealed a low swelling ratio. The higher the concentration of the initial polymer, the longer it took for it to be degraded by collagenase. The average cell viability ratio was over 92% when using the direct contact method, which suggests that the hydrogels have excellent cytocompatibility. No death, tumorigenesis, exposure of the implants, or skin necrosis associated with the subcutaneous implantation of the hydrogels was found in mice in vivo. Moreover, histological evaluation revealed the formation of a thin fibrous capsule, which suggests an acceptable biocompatibility. Furthermore, as hypothesized, it was confirmed that the biodegradability can be adjusted by changing the initial polymer concentration. Collectively, the ability to fine-tune the biodegradability of RCPhC1 hydrogels demonstrates their potential for use in various clinical applications.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hidrogeles / Colágeno Tipo I Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: J Biomater Appl Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2022 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hidrogeles / Colágeno Tipo I Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: J Biomater Appl Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2022 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido