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Profiling of humoral immune responses to norovirus in children across Europe.
Villabruna, Nele; Izquierdo-Lara, Ray W; Schapendonk, Claudia M E; de Bruin, Erwin; Chandler, Felicity; Thao, Tran Thi Nhu; Westerhuis, Brenda M; van Beek, Janko; Sigfrid, Louise; Giaquinto, Carlo; Goossens, Herman; Bielicki, Julia A; Kohns Vasconcelos, Malte; Fraaij, Pieter L A; Koopmans, Marion P G; de Graaf, Miranda.
Afiliación
  • Villabruna N; Department of Viroscience, Erasmus MC, Wytemaweg 80, 3015 CN, Rotterdam, The Netherlands.
  • Izquierdo-Lara RW; Department of Viroscience, Erasmus MC, Wytemaweg 80, 3015 CN, Rotterdam, The Netherlands.
  • Schapendonk CME; Department of Viroscience, Erasmus MC, Wytemaweg 80, 3015 CN, Rotterdam, The Netherlands.
  • de Bruin E; Department of Viroscience, Erasmus MC, Wytemaweg 80, 3015 CN, Rotterdam, The Netherlands.
  • Chandler F; Department of Viroscience, Erasmus MC, Wytemaweg 80, 3015 CN, Rotterdam, The Netherlands.
  • Thao TTN; Institute of Virology and Immunology (IVI), Bern, Switzerland.
  • Westerhuis BM; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
  • van Beek J; Department of Microbiology, Blavatnik Institute, Harvard Medical School, Boston, MA, USA.
  • Sigfrid L; Department of Viroscience, Erasmus MC, Wytemaweg 80, 3015 CN, Rotterdam, The Netherlands.
  • Giaquinto C; Department of Viroscience, Erasmus MC, Wytemaweg 80, 3015 CN, Rotterdam, The Netherlands.
  • Goossens H; Nuffield Department of Medicine, Centre for Tropical Medicine and Global Health, University of Oxford, Oxford, UK.
  • Bielicki JA; Division of Paediatric Infectious Diseases, Department of Women's and Children's Health, University Hospital of Padua, Padua, Italy.
  • Kohns Vasconcelos M; Laboratory of Medical Microbiology, Vaccine & Infectious Disease Institute (VAXINFECTIO), University of Antwerp, Antwerp, Belgium.
  • Fraaij PLA; Paediatric Infectious Disease Research Group, Institute for Infection and Immunity, St George's University of London, London, UK.
  • Koopmans MPG; Department of Infectious Diseases and Vaccinology, University of Basel Children's Hospital (UKBB), Basel, Switzerland.
  • de Graaf M; Paediatric Infectious Disease Research Group, Institute for Infection and Immunity, St George's University of London, London, UK.
Sci Rep ; 12(1): 14275, 2022 08 22.
Article en En | MEDLINE | ID: mdl-35995986
Norovirus is a leading cause of epidemic acute gastroenteritis. More than 30 genotypes circulate in humans, some are common, and others are only sporadically detected. Here, we investigated whether serology can be used to determine which genotypes infect children. We established a multiplex protein microarray with structural and non-structural norovirus antigens that allowed simultaneous antibody testing against 30 human GI and GII genotypes. Antibody responses of sera obtained from 287 children aged < 1 month to 5.5 years were profiled. Most specific IgG and IgA responses were directed against the GII.2, GII.3, GII.4, and GII.6 capsid genotypes. While we detected antibody responses against rare genotypes, we found no evidence for wide circulation. We also detected genotype-specific antibodies against the non-structural proteins p48 and p22 in sera of older children. In this study, we show the age-dependent antibody responses to a broad range of norovirus capsid and polymerase genotypes, which will aid in the development of vaccines.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por Caliciviridae / Norovirus / Inmunidad Humoral / Gastroenteritis Límite: Child, preschool / Humans / Infant País/Región como asunto: Europa Idioma: En Revista: Sci Rep Año: 2022 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Reino Unido
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por Caliciviridae / Norovirus / Inmunidad Humoral / Gastroenteritis Límite: Child, preschool / Humans / Infant País/Región como asunto: Europa Idioma: En Revista: Sci Rep Año: 2022 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Reino Unido