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TM4SF19-AS1 facilitates the proliferation of lung squamous cell carcinoma by recruiting WDR5 to mediate TM4SF19.
Luo, Machang; Xie, Lingyan; Su, Yonghua; Zhang, Kaijun; Liang, Rongzhang; Ma, Zhiyi; Li, Youtang.
Afiliación
  • Luo M; Department of Respiratory and Critical Care Medicine, The First Hospital of Longyan Affiliated to Fujian Medical University, Longyan City, Fujian Province, 364000, China.
  • Xie L; Department of Respiratory and Critical Care Medicine, The First Hospital of Longyan Affiliated to Fujian Medical University, Longyan City, Fujian Province, 364000, China.
  • Su Y; Department of Respiratory and Critical Care Medicine, The First Hospital of Longyan Affiliated to Fujian Medical University, Longyan City, Fujian Province, 364000, China.
  • Zhang K; Department of Respiratory and Critical Care Medicine, The First Hospital of Longyan Affiliated to Fujian Medical University, Longyan City, Fujian Province, 364000, China.
  • Liang R; Department of Respiratory and Critical Care Medicine, The First Hospital of Longyan Affiliated to Fujian Medical University, Longyan City, Fujian Province, 364000, China.
  • Ma Z; Department of Respiratory and Critical Care Medicine, The First Hospital of Longyan Affiliated to Fujian Medical University, Longyan City, Fujian Province, 364000, China.
  • Li Y; Department of Respiratory and Critical Care Medicine, The First Hospital of Longyan Affiliated to Fujian Medical University, Longyan City, Fujian Province, 364000, China. Electronic address: lyongt1@163.com.
Mol Cell Probes ; 65: 101849, 2022 10.
Article en En | MEDLINE | ID: mdl-35987447
BACKGROUND: As reported, long non-coding RNAs are a pivotal player in lung squamous cell carcinoma (LSCC) progression. We noticed the remarkably upregulated transmembrane-4-l-six-family-19 antisense RNA 1 (TM4SF19-AS1) in LSCC and further demonstrated the function it played in LSCC and the possible molecular mechanism. METHODS: Via bioinformatics approach, we evaluated TM4SF19-AS1 and TM4SF19 levels in LSCC tissue, and real-time quantitative polymerase chain reaction (qRT-PCR) and Western blot revealed their mRNA and protein levels in LSCC cells. Cell Counting Kit-8 and colony formation assays analyzed the proliferation ability of LSCC cells, and cell adhesion ability was detected via cell adhesion assay. RNA immunoprecipitation and chromatin immunoprecipitation analyzed the underlying mechanism of TM4SF19-AS1 regulating its target, while methylation-specific PCR indicated the methylation level of TM4SF19-AS1. RESULTS: TM4SF19-AS1 was markedly upregulated in LSCC. Functional assays revealed that TM4SF19-AS1 could facilitate the proliferation and adhesion of LSCC. Besides, we revealed the mechanism of TM4SF19-AS1 regulation that it directly bound to WD repeat-containing protein 5 (WDR5), and was then recruited to TM4SF19 promoter region, which activated DNA demethylation, thereby suppressing malignant LSCC progression. CONCLUSION: Our research demonstrated that TM4SF19-AS1 affected LSCC cell proliferation by recruiting WDR5 to manipulate transmembrane-4-lsix-family-member-19 (TM4SF19), which offers a new observation on LSCC pathogenesis, indicating that TM4SF19-AS1 is able to be a promising target for LSCC treatment.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carcinoma de Células Escamosas / Carcinoma de Pulmón de Células no Pequeñas / MicroARNs / ARN Largo no Codificante / Neoplasias Pulmonares Límite: Humans Idioma: En Revista: Mol Cell Probes Asunto de la revista: BIOLOGIA MOLECULAR / BIOTECNOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carcinoma de Células Escamosas / Carcinoma de Pulmón de Células no Pequeñas / MicroARNs / ARN Largo no Codificante / Neoplasias Pulmonares Límite: Humans Idioma: En Revista: Mol Cell Probes Asunto de la revista: BIOLOGIA MOLECULAR / BIOTECNOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido