Your browser doesn't support javascript.
loading
Genetic heterogeneity and predominance of blaCTX-M -15 in cefotaxime-resistant Enterobacteriaceae isolates colonizing hospitalized children in Tunisia.
Harbaoui, S; Ferjani, S; Abbassi, M S; Saidani, M; Gargueh, T; Ferjani, M; Hammi, Y; Boutiba-Ben Boubaker, I.
Afiliación
  • Harbaoui S; Faculté de Médecine de Tunis, LR99ES09 Laboratoire de recherche « Résistance aux antimicrobiens ¼, Université de Tunis El Manar, Tunis, Tunisia.
  • Ferjani S; Faculté de Médecine de Tunis, LR99ES09 Laboratoire de recherche « Résistance aux antimicrobiens ¼, Université de Tunis El Manar, Tunis, Tunisia.
  • Abbassi MS; Faculté de Médecine de Tunis, LR99ES09 Laboratoire de recherche « Résistance aux antimicrobiens ¼, Université de Tunis El Manar, Tunis, Tunisia.
  • Saidani M; Institut de la recherche vétérinaire de Tunisie, Université de Tunis El Manar, Tunis, Tunisia.
  • Gargueh T; Faculté de Médecine de Tunis, LR99ES09 Laboratoire de recherche « Résistance aux antimicrobiens ¼, Université de Tunis El Manar, Tunis, Tunisia.
  • Ferjani M; Service de Microbiologie, CHU Charles Nicolle, Tunis, Tunisia.
  • Hammi Y; Service de Pédiatrie, CHU Charles Nicolle, Tunis, Tunisia.
  • Boutiba-Ben Boubaker I; Service de Pédiatrie, CHU Charles Nicolle, Tunis, Tunisia.
Lett Appl Microbiol ; 75(6): 1460-1474, 2022 Dec.
Article en En | MEDLINE | ID: mdl-35985983
Extended-spectrum ß-lactamase (ESBL)-producing Enterobacteriaceae have emerged as important nosocomial pathogens. Community infections by these organisms have been also reported and were associated with previous intestinal colonization. We aimed to characterize cefotaxime-resistant Enterobacteriaceae (CTX-R-En) isolated from hospitalized children in a Tunisian paediatric ward. Seventy CTX-R-En isolates were collected from 227 rectal swabs from hospitalized children in a paediatric ward. Antimicrobial susceptibility testing was determined according to the EUCAST guidelines. Isolates were characterized by polymerase chain reaction (PCR, genes encoding: ESBLs, pAmpC, carbapenemases, plasmid-mediated quinolone resistance, virulence factors in Escherichia coli and Klebsiella pneumoniae isolates, occurrence of classes 1 and 2 integrons, phylogenetic groups of E. coli isolates, ERIC-PCR and PCR-based replicon typing) and conjugal transfer experiments. In total, 65 out of 227 (28·6%) hospitalized children were colonized with CTX-M-R-En, and 70 isolates were identified. Isolates were 59 ESBL-, 7 plasmidic-AmpC (pAmpC)-, 3 ESBL+pAmpC-, and one ESBL+carbapenemase producers. The following bla genes were identified: blaCTX-M-15 (n = 54), blaCTX-M-1 (n = 5), blaCTX-M-9 (n = 2), blaCTX-M-13 (n = 1) and blaCTX-M-14 (n = 1), blaCMY-2 (n = 5), blaCMY-4 (n = 4), blaACC-1 (n = 1) and blaOXA-48 (n = 1). Our results showed that hospitalized children were colonized with various CTX-R-En-producing several beta-lactamase enzymes.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cefotaxima / Enterobacteriaceae Tipo de estudio: Prognostic_studies Límite: Child / Humans País/Región como asunto: Africa Idioma: En Revista: Lett Appl Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Túnez Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cefotaxima / Enterobacteriaceae Tipo de estudio: Prognostic_studies Límite: Child / Humans País/Región como asunto: Africa Idioma: En Revista: Lett Appl Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Túnez Pais de publicación: Reino Unido