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Antibacterial potency of cytocompatible chitosan-decorated biogenic silver nanoparticles and molecular insights towards cell-particle interaction.
Samanta, Sovan; Banerjee, Jhimli; Das, Balaram; Mandal, Jayanta; Chatterjee, Soumendranath; Ali, Kazi Monjur; Sinha, Sangram; Giri, Biplab; Ghosh, Totan; Dash, Sandeep Kumar.
Afiliación
  • Samanta S; Department of Physiology, University of Gour Banga, Malda 732103, West Bengal, India.
  • Banerjee J; Department of Physiology, University of Gour Banga, Malda 732103, West Bengal, India.
  • Das B; Department of Physiology, Belda College, Paschim Medinipur 721424, West Bengal, India.
  • Mandal J; Department of Botany, Vivekananda Mahavidyalaya, Haripal, Hooghly 712405, West Bengal, India.
  • Chatterjee S; Department of Zoology, The University of Burdwan, 713104, West Bengal, India.
  • Ali KM; Department of Nutrition, M.U.C Women's College, Purba Bardhaman 713104, West Bengal, India.
  • Sinha S; Department of Botany, Vivekananda Mahavidyalaya, Haripal, Hooghly 712405, West Bengal, India.
  • Giri B; Department of Physiology, University of Gour Banga, Malda 732103, West Bengal, India.
  • Ghosh T; Department of Applied Chemistry, Maulana Abul Kalam Azad University of Technology, 741249, West Bengal, India.
  • Dash SK; Department of Physiology, University of Gour Banga, Malda 732103, West Bengal, India. Electronic address: deep.vu@gmail.com.
Int J Biol Macromol ; 219: 919-939, 2022 Oct 31.
Article en En | MEDLINE | ID: mdl-35961557
In the study, leaf extract of Carica papaya was utilized for the biogenic fabrication process of chitosan functionalized silver nanoparticles (Ag-Chito NPs). HRTEM analysis revealed that the fabricated Ag-Chito NPs was spherical in shape, with an average particle size of 13.31 (±0.07) nm. FTIR, UV-Vis, DLS, and other characterizations were also performed to analyze the diverse physicochemical properties of the particles. The antibacterial potency of the synthesized Ag-Chito NPs was tested against the two clinically isolated multidrug resistant uropathogenic bacterial strains, i.e. MLD 2 (Escherichia coli) and MLD 4 (Staphylococcus aureus) through MIC, MBC, time and concentration dependent killing kinetic assay, inhibition of biofilm formation assay, fluorescence and SEM imaging. Significantly, Ag-Chito NPs showed the highest sensitivity against the MLD 2 (MIC value of 12.5 µg/mL) strain, as compared to the MLD 4 (MIC value of 15 µg/mL) strain. From the hemolysis assay, it was revealed that Ag-Chito NPs exerted no significant toxicity up to 50 µg/mL against healthy human blood cells. Additionally, in silico analysis of chitosan (functionalized on the surface of AgNPs) and bacterial cell membrane protein also evidently suggested a strong interaction between Ag-Chito NPs and bacterial cells, which might be responsible for bacterial cell death.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Quitosano / Nanopartículas del Metal Límite: Humans Idioma: En Revista: Int J Biol Macromol Año: 2022 Tipo del documento: Article País de afiliación: India Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Quitosano / Nanopartículas del Metal Límite: Humans Idioma: En Revista: Int J Biol Macromol Año: 2022 Tipo del documento: Article País de afiliación: India Pais de publicación: Países Bajos