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Exploration of biomimetic poly(γ-benzyl-L-glutamate) fibrous scaffolds for corneal nerve regeneration.
Ma, Tien-Li; Yang, Shang-Chih; Cheng, Ting; Chen, Mei-Yun; Wu, Jo-Hsuan; Liao, Shu-Lang; Chen, Wei-Li; Su, Wei-Fang.
Afiliación
  • Ma TL; Department of Materials Science and Engineering, National Taiwan University, Taipei, Taiwan. suwf@ntu.edu.tw.
  • Yang SC; Department of Ophthalmology, National Taiwan University College of Medicine, Taipei, Taiwan. chenweili@ntu.edu.tw.
  • Cheng T; Department of Ophthalmology, National Taiwan University College of Medicine, Taipei, Taiwan. chenweili@ntu.edu.tw.
  • Chen MY; Department of Ophthalmology, National Taiwan University Hospital, Taipei, Taiwan.
  • Wu JH; Shiley Eye Institute and Viterbi Family Department of Ophthalmology, University of California, San Diego, California, USA.
  • Liao SL; Department of Ophthalmology, National Taiwan University College of Medicine, Taipei, Taiwan. chenweili@ntu.edu.tw.
  • Chen WL; Department of Ophthalmology, National Taiwan University Hospital, Taipei, Taiwan.
  • Su WF; Department of Ophthalmology, National Taiwan University College of Medicine, Taipei, Taiwan. chenweili@ntu.edu.tw.
J Mater Chem B ; 10(33): 6372-6379, 2022 08 24.
Article en En | MEDLINE | ID: mdl-35950376
Poly(γ-benzyl-L-glutamate) (PBG) made biomimetic scaffolds are explored as candidate materials for corneal nerve regeneration and neurotrophic keratopathy treatment. The PBG with built-in neurotransmitter glutamate was synthesized and fabricated into 3D fibrous scaffolds containing aligned fibers using electrospinning. In in vitro experiments, primary mouse trigeminal ganglia (TG) cells were used. Immunohistochemistry (IHC) analysis shows that TG cells cultured on PBG have no cytotoxic response for 21 days. Without any nerve growth factor, TG cells have the longest neurite length of 225.3 µm in the PBG group and 1.3 times the average length as compared with the polycaprolactone and no scaffold groups. Also, aligned fibers guide the neurite growth and extension unidirectionally. In vivo assays were carried out by intracorneal implantation of PBG on clinical New Zealand rabbits. The external eye photos and in vivo confocal microscopy (IVCM) show a low immune response. The corneal neural markers (ßIII tubulin and SMI312) in the IHC analysis are consistent with the position stained by glutamate of implanted scaffolds, indicating that PBG induces neurogenesis. PBG exhibits mechanical stiffness to resist material deformation possibly caused by surgical operations. The results of this study demonstrate that PBG is suitable for corneal nerve regeneration and the treatment of neurotrophic keratopathy.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácido Glutámico / Andamios del Tejido Límite: Animals Idioma: En Revista: J Mater Chem B Año: 2022 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácido Glutámico / Andamios del Tejido Límite: Animals Idioma: En Revista: J Mater Chem B Año: 2022 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Reino Unido