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iChip increases the success of cultivation of TBT-resistant and TBT-degrading bacteria from estuarine sediment.
Polrot, A; Kirby, J R; Olorunniji, F J; Birkett, J W; Sharples, G P.
Afiliación
  • Polrot A; Liverpool John Moores University, Byrom Street, Liverpool, L3 3AF, UK.
  • Kirby JR; Liverpool John Moores University, Byrom Street, Liverpool, L3 3AF, UK. J.R.Kirby@ljmu.ac.uk.
  • Olorunniji FJ; Liverpool John Moores University, Byrom Street, Liverpool, L3 3AF, UK.
  • Birkett JW; Liverpool John Moores University, Byrom Street, Liverpool, L3 3AF, UK.
  • Sharples GP; Liverpool John Moores University, Byrom Street, Liverpool, L3 3AF, UK.
World J Microbiol Biotechnol ; 38(10): 180, 2022 Aug 11.
Article en En | MEDLINE | ID: mdl-35948836
Standard methods of microbial cultivation only enable the isolation of a fraction of the total environmental bacteria. Numerous techniques have been developed to increase the success of isolation and cultivation in the laboratory, some of which derive from diffusion chambers. In a diffusion chamber, environmental bacteria in agar medium are put back in the environment to grow as close to their natural conditions as possible, only separated from the environment by semi-permeable membranes. In this study, the iChip, a device that possesses hundreds of mini diffusion chambers, was used to isolate tributyltin (TBT) resistant and degrading bacteria. IChip was shown to be efficient at increasing the number of cultivable bacteria compared to standard methods. TBT-resistant strains belonging to Oceanisphaera sp., Pseudomonas sp., Bacillus sp. and Shewanella sp. were identified from Liverpool Dock sediment. Among the isolates in the present study, only members of Pseudomonas sp. were able to use TBT as a sole carbon source. It is the first time that members of the genus Oceanisphaera have been shown to be TBT-resistant. Although iChip has been used in the search for molecules of biomedical interest here we demonstrate its promising application in bioremediation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Compuestos de Trialquiltina / Contaminantes Químicos del Agua Idioma: En Revista: World J Microbiol Biotechnol Año: 2022 Tipo del documento: Article Pais de publicación: Alemania
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Compuestos de Trialquiltina / Contaminantes Químicos del Agua Idioma: En Revista: World J Microbiol Biotechnol Año: 2022 Tipo del documento: Article Pais de publicación: Alemania