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Zona pellucida removal by acid Tyrode's solution affects pre- and post-implantation development and gene expression in mouse embryos†.
Fan, Weihong; Huang, Tengda; Wu, Tian; Bai, Hanako; Kawahara, Manabu; Takahashi, Masashi.
Afiliación
  • Fan W; Graduate School of Agriculture, Hokkaido University, Hokkaido, Japan.
  • Huang T; Department of Liver Surgery & Liver Transplantation, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, China.
  • Wu T; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Animal Science and Technology, Guangxi University, Nanning, P.R. China.
  • Bai H; Graduate School of Agriculture, Hokkaido University, Hokkaido, Japan.
  • Kawahara M; Research Faculty of Agriculture, Hokkaido University, Hokkaido, Japan.
  • Takahashi M; Graduate School of Agriculture, Hokkaido University, Hokkaido, Japan.
Biol Reprod ; 107(5): 1228-1241, 2022 11 14.
Article en En | MEDLINE | ID: mdl-35948000
The zona pellucida plays a crucial role in the process of fertilization to early embryonic development, including cellular arrangement and communication between blastomeres. However, little is known regarding the role of the zona pellucida in pre- and post-implantation embryonic development associated with gene expression. We investigated the effect of zona pellucida removal on pre- and post-implantation development of mouse embryos. After zona pellucida removal of two-cell stage embryos was performed by acid Tyrode's solution, which is commonly used for zona pellucida treatment, compaction occurred earlier in zona pellucida-free than zona pellucida-intact embryos. In addition, the expression of differentiation-related genes in the inner cell mass and trophectoderm was significantly altered in zona pellucida-free blastocyst compared with zona pellucida-intact embryos. After embryo transfer, the rate of implantation and live fetuses was lower in zona pellucida-free embryos than in control embryos, whereas the fetal weight at E17.5 was not different. However, placental weight significantly increased in zona pellucida-free embryos. RNA-sequencing analysis of the placenta showed that a total of 473 differentially expressed genes significantly influenced the biological process. The present study suggests that zona pellucida removal by acid Tyrode's solution at the two-cell stage not only disturbs the expression pattern of inner cell mass-/trophectoderm-related genes but affects the post-implantation development of mouse embryos. Overall, this study provides deeper insight into the role of the zona pellucida during early embryonic development and the viability of post-implantation development.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Placenta / Zona Pelúcida Límite: Animals / Pregnancy Idioma: En Revista: Biol Reprod Año: 2022 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Placenta / Zona Pelúcida Límite: Animals / Pregnancy Idioma: En Revista: Biol Reprod Año: 2022 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos