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Hydrogen­deuterium exchange mass spectrometry to study interactions and conformational changes of proteins in paints.
Galluzzi, Francesca; Chaignepain, Stéphane; Arslanoglu, Julie; Tokarski, Caroline.
Afiliación
  • Galluzzi F; Institute of Chemistry and Biology of Membrane and NanoObjects, UMR CNRS 5248, Proteome Platform, University of Bordeaux, Bordeaux, France.
  • Chaignepain S; Institute of Chemistry and Biology of Membrane and NanoObjects, UMR CNRS 5248, Proteome Platform, University of Bordeaux, Bordeaux, France.
  • Arslanoglu J; Department of Scientific Research, The Metropolitan Museum of Art, New York, USA.
  • Tokarski C; Institute of Chemistry and Biology of Membrane and NanoObjects, UMR CNRS 5248, Proteome Platform, University of Bordeaux, Bordeaux, France. Electronic address: caroline.tokarski@u-bordeaux.fr.
Biophys Chem ; 289: 106861, 2022 10.
Article en En | MEDLINE | ID: mdl-35940022
Little is known about structural alterations of proteins within the polymeric films of paints. For the first time, hydrogen­deuterium exchange mass spectrometry (HDX-MS) was implemented to explore the conformational alterations of proteins resulting from their interaction with inorganic pigments within the early stages of the paint film formation. Intact protein analysis and bottom-up electrospray-ionisation mass spectrometry strategies combined with progressively increasing deuterium incubation times were used to compare the protein structures of the model protein hen egg-white lysozyme (HEWL) extracted from newly dried non-pigmented films and newly dried films made from a freshly made mixture of HEWL with lead white pigment (2PbCO3 Pb(OH)2). The action of other pigments was also investigated, expanding the HDX study with a global approach to paint models of HEWL mixed with zinc white (ZnO), cinnabar (HgS) and red lead (Pb3O4) pigments. The results show structural modifications of HEWL induced by the interaction with the pigment metal ions during the paint formulation after drying and prior to ageing. Both the charge distribution of HEWL proteoforms, its oxidation rate and its deuterium absorption rate, were influenced by the pigment type, providing the first insights into the correlation of pigment type/metal cation to specific chemistries related to protein stability.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Medición de Intercambio de Deuterio / Espectrometría de Masas de Intercambio de Hidrógeno-Deuterio Idioma: En Revista: Biophys Chem Año: 2022 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Medición de Intercambio de Deuterio / Espectrometría de Masas de Intercambio de Hidrógeno-Deuterio Idioma: En Revista: Biophys Chem Año: 2022 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Países Bajos