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Analysis of NGS data from Peruvian Loxosceles laeta spider venom gland reveals toxin diversity.
Medina-Santos, Raíssa; Fernandes Costa, Tamara G; Silva de Assis, Thamyres C; Kalapothakis, Yan; de Almeida Lima, Sabrina; do Carmo, Anderson Oliveira; Gonzalez-Kozlova, Edgar E; Kalapothakis, Evanguedes; Chávez-Olórtegui, Carlos; Guerra-Duarte, Clara.
Afiliación
  • Medina-Santos R; Biochemistry and Immunology Department, Federal University of Minas Gerais, Brazil; Genetic, Ecology and Evolution Department, Federal University of Minas Gerais, Brazil.
  • Fernandes Costa TG; Biochemistry and Immunology Department, Federal University of Minas Gerais, Brazil.
  • Silva de Assis TC; Biochemistry and Immunology Department, Federal University of Minas Gerais, Brazil.
  • Kalapothakis Y; Genetic, Ecology and Evolution Department, Federal University of Minas Gerais, Brazil.
  • de Almeida Lima S; Biochemistry and Immunology Department, Federal University of Minas Gerais, Brazil.
  • do Carmo AO; Genetic, Ecology and Evolution Department, Federal University of Minas Gerais, Brazil.
  • Gonzalez-Kozlova EE; Department of Genetics and Genomic Sciences, Icahn School for Data Science and Genomic Technology, New York, United States of America.
  • Kalapothakis E; Genetic, Ecology and Evolution Department, Federal University of Minas Gerais, Brazil.
  • Chávez-Olórtegui C; Biochemistry and Immunology Department, Federal University of Minas Gerais, Brazil.
  • Guerra-Duarte C; Ezequiel Dias Foundation, Brazil. Electronic address: clara.duarte@funed.mg.gov.br.
Comp Biochem Physiol Part D Genomics Proteomics ; 43: 101017, 2022 09.
Article en En | MEDLINE | ID: mdl-35932519
Accidents involving spiders from the genus Loxosceles cause medical emergencies in several countries of South America. The species Loxosceles laeta is ubiquitously present in Peru and is responsible for severe accidents in this country. To further characterize L. laeta venom components and to unveil possible variations in the Peruvian population, we provide an overview of the toxins-related transcripts present in the venom gland of Peruvian L. laeta. A dataset from a cDNA library previously sequenced by MiSeq sequencer (Illumina) was re-analyzed and the obtained data was compared with available sequences from Loxosceles toxins. Phospholipase-D represent the majority (69,28 %) of the transcripts related to venom toxins, followed by metalloproteases (20,72 %), sicaritoxins (6,03 %), serine-proteases (2,28 %), hyaluronidases (1,80 %) and Translationally Controlled Tumor Protein (TCTP) (0,56 %). New sequences of phospholipases D,sicaritoxins, hyaluronidase, TCTP and serine proteinases were described. Differences between the here-described toxin sequences and others, previously identified in venom glands from other spiders, were visualized upon sequence alignments. In addition, an in vitro hyaluronidase activity assay was also performed to complement comparisons between Peruvian and Brazilian L. laeta venom enzymatic activities, revealing a superior activity in the venom from Brazilian specimens. These new data provide a molecular basis that can help to explain the difference in toxicity among L. laeta venoms from different countries in South America.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Venenos de Araña / Hialuronoglucosaminidasa Tipo de estudio: Prognostic_studies Límite: Animals País/Región como asunto: America do sul / Peru Idioma: En Revista: Comp Biochem Physiol Part D Genomics Proteomics Asunto de la revista: BIOLOGIA / GENETICA Año: 2022 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Venenos de Araña / Hialuronoglucosaminidasa Tipo de estudio: Prognostic_studies Límite: Animals País/Región como asunto: America do sul / Peru Idioma: En Revista: Comp Biochem Physiol Part D Genomics Proteomics Asunto de la revista: BIOLOGIA / GENETICA Año: 2022 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos