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Gemini surfactant-based nanoparticles T-box1 gene delivery as a novel approach to promote epithelial stem cells differentiation and dental enamel formation.
Mohabatpour, Fatemeh; Al-Dulaymi, Mays; Lobanova, Liubov; Scutchings, Brittany; Papagerakis, Silvana; Badea, Ildiko; Chen, Xiongbiao; Papagerakis, Petros.
Afiliación
  • Mohabatpour F; Division of Biomedical Engineering, University of Saskatchewan, 57 Campus Dr., S7N 5A9 SK, Canada; College of Dentistry, University of Saskatchewan, 105 Wiggins Rd, S7N 5E4, SK, Canada.
  • Al-Dulaymi M; College of Pharmacy and Nutrition, University of Saskatchewan, 107 Wiggins Rd, S7N 5E5, SK, Canada.
  • Lobanova L; College of Dentistry, University of Saskatchewan, 105 Wiggins Rd, S7N 5E4, SK, Canada.
  • Scutchings B; College of Pharmacy and Nutrition, University of Saskatchewan, 107 Wiggins Rd, S7N 5E5, SK, Canada.
  • Papagerakis S; Division of Biomedical Engineering, University of Saskatchewan, 57 Campus Dr., S7N 5A9 SK, Canada; Department of Surgery, College of Medicine, University of Saskatchewan, 107 Wiggins Rd B419, S7N 0W8, SK, Canada; Department of Otolaryngology, College of Medicine, University of Michigan, Ann Arbor, M
  • Badea I; College of Pharmacy and Nutrition, University of Saskatchewan, 107 Wiggins Rd, S7N 5E5, SK, Canada.
  • Chen X; Division of Biomedical Engineering, University of Saskatchewan, 57 Campus Dr., S7N 5A9 SK, Canada; Department of Mechanical Engineering, University of Saskatchewan, 57 Campus Dr., S7N 5A9 SK, Canada. Electronic address: xbc719@usask.ca.
  • Papagerakis P; Division of Biomedical Engineering, University of Saskatchewan, 57 Campus Dr., S7N 5A9 SK, Canada; College of Dentistry, University of Saskatchewan, 105 Wiggins Rd, S7N 5E4, SK, Canada. Electronic address: petros.papagerakis@usask.ca.
Biomater Adv ; 137: 212844, 2022 Jun.
Article en En | MEDLINE | ID: mdl-35929273
Enamel is the highest mineralized tissue in the body protecting teeth from external stimuli, infections, and injuries. Enamel lacks the ability to self-repair due to the absence of enamel-producing cells in the erupted teeth. Here, we reported a novel approach to promote enamel-like tissue formation via the delivery of a key ameloblast inducer, T-box1 gene, into a rat dental epithelial stem cell line, HAT-7, using non-viral gene delivery systems based on cationic lipids. We comparatively assessed the lipoplexes prepared from glycyl-lysine-modified gemini surfactants and commercially available 1,2-dioleoyl-3-trimethylammonium-propane lipids at three nitrogen-to phosphate (N/P) ratios of 2.5, 5 and 10. Our findings revealed that physico-chemical characteristics and biological activities of the gemini surfactant-based lipoplexes with a N/P ratio of 5 provide the most optimal outcomes among those examined. HAT-7 cells were transfected with T-box1 gene using the optimal formulation then cultured in conventional 2D cell culture systems. Ameloblast differentiation, mineralization, bio-enamel interface and structure were assessed at different time points over 28 days. Our results showed that our gemini transfection system provides superior gene expression compared to the benchmark agent, while keeping low cytotoxicity levels. T-box1-transfected HAT-7 cells strongly expressed markers of secretory and maturation stages of the ameloblasts, deposited minerals, and produced enamel-like crystals when compared to control cells. Taken together, our gemini surfactant-based T-box1 gene delivery system is effective to accelerate and guide ameloblastic differentiation of dental epithelial stem cells and promote enamel-like tissue formation. This study would represent a significant advance towards the tissue engineering and regeneration of dental enamel.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Surfactantes Pulmonares / Nanopartículas Límite: Animals Idioma: En Revista: Biomater Adv Año: 2022 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Surfactantes Pulmonares / Nanopartículas Límite: Animals Idioma: En Revista: Biomater Adv Año: 2022 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Países Bajos