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Standardized excitable elements for scalable engineering of far-from-equilibrium chemical networks.
Schaffter, Samuel W; Chen, Kuan-Lin; O'Brien, Jackson; Noble, Madeline; Murugan, Arvind; Schulman, Rebecca.
Afiliación
  • Schaffter SW; Department of Chemical & Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA.
  • Chen KL; Department of Chemical & Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA.
  • O'Brien J; Department of Physics, University of Chicago, Chicago, IL, USA.
  • Noble M; Department of Chemical & Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA.
  • Murugan A; Department of Physics, University of Chicago, Chicago, IL, USA.
  • Schulman R; Department of Chemical & Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA. rschulm3@jhu.edu.
Nat Chem ; 14(11): 1224-1232, 2022 11.
Article en En | MEDLINE | ID: mdl-35927329
Engineered far-from-equilibrium synthetic chemical networks that pulse or switch states in response to environmental signals could precisely regulate the kinetics of chemical synthesis or self-assembly. Currently, such networks must be extensively tuned to compensate for the different activities of and unintended reactions between a network's various chemical components. Modular elements with standardized performance could be used to rapidly construct networks with designed functions. Here we develop standardized excitable chemical regulatory elements, termed genelets, and use them to construct complex in vitro transcriptional networks. We develop a protocol for identifying >15 interchangeable genelet elements with uniform performance and minimal crosstalk. These elements can be combined to engineer feedforward and feedback modules whose dynamics match those predicted by a simple kinetic model. Modules can then be rationally integrated and organized into networks that produce tunable temporal pulses and act as multistate switchable memories. Standardized genelet elements, and the workflow to identify more, should make engineering complex far-from-equilibrium chemical dynamics routine.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Redes Reguladoras de Genes Tipo de estudio: Prognostic_studies Idioma: En Revista: Nat Chem Asunto de la revista: QUIMICA Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Redes Reguladoras de Genes Tipo de estudio: Prognostic_studies Idioma: En Revista: Nat Chem Asunto de la revista: QUIMICA Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido