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Structural and genetic insights into a poly-γ-glutamic acid with in vitro antioxidant activity of Bacillus velezensis VCN56.
Quach, Ngoc Tung; Vu, Thi Hanh Nguyen; Nguyen, Thi Thu An; Ha, Hoang; Ho, Phu-Ha; Chu-Ky, Son; Nguyen, Lan-Huong; Van Nguyen, Hai; Thanh, Thi Thu Thuy; Nguyen, Ngoc Anh; Chu, Hoang Ha; Phi, Quyet-Tien.
Afiliación
  • Quach NT; Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, 100000, Vietnam.
  • Vu THN; Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi, 100000, Vietnam.
  • Nguyen TTA; Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, 100000, Vietnam.
  • Ha H; Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi, 100000, Vietnam.
  • Ho PH; Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, 100000, Vietnam.
  • Chu-Ky S; Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, 100000, Vietnam.
  • Nguyen LH; School of Biotechnology and Food Technology, Hanoi University of Science and Technology, Hanoi, 100000, Vietnam.
  • Van Nguyen H; School of Biotechnology and Food Technology, Hanoi University of Science and Technology, Hanoi, 100000, Vietnam.
  • Thanh TTT; School of Biotechnology and Food Technology, Hanoi University of Science and Technology, Hanoi, 100000, Vietnam.
  • Nguyen NA; School of Biotechnology and Food Technology, Hanoi University of Science and Technology, Hanoi, 100000, Vietnam.
  • Chu HH; Institute of Chemistry, Vietnam Academy of Science and Technology, Hanoi, 100000, Vietnam.
  • Phi QT; Institute of Chemistry, Vietnam Academy of Science and Technology, Hanoi, 100000, Vietnam.
World J Microbiol Biotechnol ; 38(10): 173, 2022 Aug 03.
Article en En | MEDLINE | ID: mdl-35920928
Poly-γ­glutamic acid (γ­PGA) produced by Bacillus species is a natural biopolymer, which is widely used in various fields including food, pharmaceuticals, and cosmetics. In this study, the screening of 19 Bacillus isolates derived from traditionally fermented foods revealed that Bacillus velezensis VCN56 was the most potent γ­PGA producer. The maximum concentration of crude γ­PGA was 32.9 ± 1.5 g/L in the PGA-3 medium containing glycerol, citric acid, sodium glutamate, NH4Cl, and starch. The resulting γ-PGA was purified and then characterized by HPLC, FTIR, and 1H-NMR analyses. Molecular weight of purified γ­PGA was estimated to be 98 kDa with a polydisperse index of 2.04. Notably, the pure γ­PGA showed significant in vitro antioxidant scavenging activities against 1,1-diphenyl-2-picrylhydrazyl (72.0 ± 1.5%), hydroxyl (81.0 ± 0.6%), and superoxide (43.9 ± 0.8%) radicals at the concentration of 4 mg/mL. Using whole-genome sequencing, the genetic organization of pgs operon responsible for γ­PGA biosynthesis in B. velezensis VCN56 differs from those in other Bacillus genomes. Further genome analysis revealed metabolic pathways for γ-PGA production and degradation. For the first time, the present study provides a better understanding of γ-PGA with a promising antioxidant activity produced by B. velezensis at the phenotypic, biochemical, and genomic levels, which hold potential applications in the foods, cosmetics, and pharmaceutical industries.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacillus / Antioxidantes Idioma: En Revista: World J Microbiol Biotechnol Año: 2022 Tipo del documento: Article País de afiliación: Vietnam Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacillus / Antioxidantes Idioma: En Revista: World J Microbiol Biotechnol Año: 2022 Tipo del documento: Article País de afiliación: Vietnam Pais de publicación: Alemania