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In vitro modeling of amyotrophic lateral sclerosis with induced pluripotent stem cell technology-derived cell line ORIONi002-A.
Strnadel, Jan; Dumortier, Hugo M; Hajduchova, Dominika; Zahumenska, Romana; Nosal, Vladimir; Smolar, Marek; Marcinek, Juraj; Kalman, Michal; Mersakova, Sandra; Brany, Dusan; Juhas, Stefan; Juhasova, Jana; Studenovska, Hana; Mitruskova, Barbora; Suroviakova, Stanislava; Novakova, Slavomira; Skovierova, Henrieta; Kurca, Egon; Pecova, Renata; Plank, Lukas; Halasova, Erika.
Afiliación
  • Strnadel J; Biomedical Centre Martin JFM CU, Jessenius Faculty of Medicine in Martin (JFM CU), Comenius University in Bratislava, Slovakia. Electronic address: jan.strnadel@uniba.sk.
  • Dumortier HM; Biomedical Centre Martin JFM CU, Jessenius Faculty of Medicine in Martin (JFM CU), Comenius University in Bratislava, Slovakia.
  • Hajduchova D; Department of Pathological Physiology, Jessenius Faculty of Medicine in Martin (JFM CU), Comenius University in Bratislava, Slovakia.
  • Zahumenska R; Biomedical Centre Martin JFM CU, Jessenius Faculty of Medicine in Martin (JFM CU), Comenius University in Bratislava, Slovakia.
  • Nosal V; Clinic of Neurology, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, University Hospital, Martin, Slovakia.
  • Smolar M; Clinic of Surgery and Transplant Center, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, University Hospital, Martin, Slovakia.
  • Marcinek J; Department of Pathological Anatomy, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, University Hospital, Martin, Slovakia.
  • Kalman M; Department of Pathological Anatomy, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, University Hospital, Martin, Slovakia.
  • Mersakova S; Biomedical Centre Martin JFM CU, Jessenius Faculty of Medicine in Martin (JFM CU), Comenius University in Bratislava, Slovakia.
  • Brany D; Biomedical Centre Martin JFM CU, Jessenius Faculty of Medicine in Martin (JFM CU), Comenius University in Bratislava, Slovakia.
  • Juhas S; PIGMOD Centre, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, v.v.i., Libechov, Czech Republic.
  • Juhasova J; PIGMOD Centre, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, v.v.i., Libechov, Czech Republic.
  • Studenovska H; Department of Biomaterials and Bioanalogous Systems Institute of Macromolecular Chemistry, Czech Academy of Sciences, Prague, Czech Republic.
  • Mitruskova B; Department of Clinical Genetics, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, University Hospital, Martin, Slovakia.
  • Suroviakova S; Clinic of Children and Adolescents, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, University Hospital, Martin, Slovakia.
  • Novakova S; Biomedical Centre Martin JFM CU, Jessenius Faculty of Medicine in Martin (JFM CU), Comenius University in Bratislava, Slovakia.
  • Skovierova H; Biomedical Centre Martin JFM CU, Jessenius Faculty of Medicine in Martin (JFM CU), Comenius University in Bratislava, Slovakia.
  • Kurca E; Clinic of Neurology, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, University Hospital, Martin, Slovakia.
  • Pecova R; Department of Pathological Physiology, Jessenius Faculty of Medicine in Martin (JFM CU), Comenius University in Bratislava, Slovakia.
  • Plank L; Department of Pathological Anatomy, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, University Hospital, Martin, Slovakia.
  • Halasova E; Biomedical Centre Martin JFM CU, Jessenius Faculty of Medicine in Martin (JFM CU), Comenius University in Bratislava, Slovakia.
Stem Cell Res ; 63: 102870, 2022 08.
Article en En | MEDLINE | ID: mdl-35907349
We present here a new iPS cell line for modeling sporadic form of ALS. Cell line was generated by reprogramming skin fibroblasts isolated with explant culture technology from skin biopsy, donated by ALS patient. For reprogramming, polycistronic self-replicating RNA vector was used and derived iPS cells were characterized by immunocytochemistry and FACS (pluripotent factors expression), karyotyping, STR fingerprinting analysis and in vitro differentiation assay. New cell line showed normal (46, XY) karyotype and differentiated in vitro into cells from three germ layers. STR analysis proved the origin and originality of the cell line.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Pluripotentes Inducidas / Esclerosis Amiotrófica Lateral Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Stem Cell Res Año: 2022 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Pluripotentes Inducidas / Esclerosis Amiotrófica Lateral Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Stem Cell Res Año: 2022 Tipo del documento: Article Pais de publicación: Reino Unido