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Determination of human insulin and its six therapeutic analogues by capillary electrophoresis - mass spectrometry.
Hamidli, Narmin; Pajaziti, Blerta; Andrási, Melinda; Nagy, Cynthia; Gáspár, Attila.
Afiliación
  • Hamidli N; Department of Inorganic and Analytical Chemistry, University of Debrecen, Egyetem tér 1, Debrecen H-4032, Hungary.
  • Pajaziti B; Department of Inorganic and Analytical Chemistry, University of Debrecen, Egyetem tér 1, Debrecen H-4032, Hungary.
  • Andrási M; Department of Inorganic and Analytical Chemistry, University of Debrecen, Egyetem tér 1, Debrecen H-4032, Hungary.
  • Nagy C; Department of Inorganic and Analytical Chemistry, University of Debrecen, Egyetem tér 1, Debrecen H-4032, Hungary.
  • Gáspár A; Department of Inorganic and Analytical Chemistry, University of Debrecen, Egyetem tér 1, Debrecen H-4032, Hungary. Electronic address: gaspar@science.unideb.hu.
J Chromatogr A ; 1678: 463351, 2022 Aug 16.
Article en En | MEDLINE | ID: mdl-35905683
In this work, human insulin and its 6 analogues were separated and determined using CZE-MS. Three different capillaries (bare fused silica, successive multiple ionic-polymer layer (SMIL) and static linear polyacrylamide (LPA) coated) were compared based on their separation performances in their optimal operating conditions. Coated capillaries demonstrated slightly better separation of the components, although some components showed wide, distorted peaks. The highest plate number could be obtained in the SMIL capillary (192 000/m). For UV and ESI-MS detection relatively similar LOD values were obtained (0.3-1.2 mg/L and 1.0-3.4 mg/L, respectively). The application of MS detection provided useful structural information and unambiguous identification for insulins having similar or the same molecular mass. This work is considered to be important not only for the investigation of insulins but also for its potential contribution to the top-down analysis of proteins using CE-MS.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas / Electroforesis Capilar Límite: Humans Idioma: En Revista: J Chromatogr A Año: 2022 Tipo del documento: Article País de afiliación: Hungria Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas / Electroforesis Capilar Límite: Humans Idioma: En Revista: J Chromatogr A Año: 2022 Tipo del documento: Article País de afiliación: Hungria Pais de publicación: Países Bajos