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Olfactory swab sampling optimization for α-synuclein aggregate detection in patients with Parkinson's disease.
Bongianni, Matilde; Catalan, Mauro; Perra, Daniela; Fontana, Elena; Janes, Francesco; Bertolotti, Claudio; Sacchetto, Luca; Capaldi, Stefano; Tagliapietra, Matteo; Polverino, Paola; Tommasini, Valentina; Bellavita, Giulia; Kachoie, Elham Ataie; Baruca, Roberto; Bernardini, Andrea; Valente, Mariarosaria; Fiorini, Michele; Bronzato, Erika; Tamburin, Stefano; Bertolasi, Laura; Brozzetti, Lorenzo; Cecchini, Maria Paola; Gigli, Gianluigi; Monaco, Salvatore; Manganotti, Paolo; Zanusso, Gianluigi.
Afiliación
  • Bongianni M; Department of Neurosciences, Biomedicine, and Movement Sciences, Policlinico G. B. Rossi, University of Verona, 37134, Verona, Italy.
  • Catalan M; Neurology Unit, Department of Medicine, Surgery and Health Sciences, Ospedale Cattinara, University of Trieste, 34128, Trieste, Italy.
  • Perra D; Department of Neurosciences, Biomedicine, and Movement Sciences, Policlinico G. B. Rossi, University of Verona, 37134, Verona, Italy.
  • Fontana E; Department of Neurosciences, Biomedicine, and Movement Sciences, Policlinico G. B. Rossi, University of Verona, 37134, Verona, Italy.
  • Janes F; Neurology Unit, University of Udine Academic Hospital, 33100, Udine, Italy.
  • Bertolotti C; Neurology Unit, Department of Medicine, Surgery and Health Sciences, Ospedale Cattinara, University of Trieste, 34128, Trieste, Italy.
  • Sacchetto L; Department of Surgical Sciences, Dentistry, Gynecology and Pediatrics, University of Verona, 37134, Verona, Italy.
  • Capaldi S; Biocrystallography Laboratory, Department of Biotechnology, University of Verona, 37134, Verona, Italy.
  • Tagliapietra M; Department of Neurosciences, Biomedicine, and Movement Sciences, Policlinico G. B. Rossi, University of Verona, 37134, Verona, Italy.
  • Polverino P; Neurology Unit, Department of Medicine, Surgery and Health Sciences, Ospedale Cattinara, University of Trieste, 34128, Trieste, Italy.
  • Tommasini V; Neurology Unit, Department of Medicine, Surgery and Health Sciences, Ospedale Cattinara, University of Trieste, 34128, Trieste, Italy.
  • Bellavita G; Neurology Unit, Department of Medicine, Surgery and Health Sciences, Ospedale Cattinara, University of Trieste, 34128, Trieste, Italy.
  • Kachoie EA; Biocrystallography Laboratory, Department of Biotechnology, University of Verona, 37134, Verona, Italy.
  • Baruca R; Otolaryngology Unit, Department of Medicine, Surgery and Health Sciences, Ospedale Cattinara, University of Trieste, 34128, Trieste, Italy.
  • Bernardini A; Neurology Unit, University of Udine Academic Hospital, 33100, Udine, Italy.
  • Valente M; Neurology Unit, University of Udine Academic Hospital, 33100, Udine, Italy.
  • Fiorini M; Department of Neurosciences, Biomedicine, and Movement Sciences, Policlinico G. B. Rossi, University of Verona, 37134, Verona, Italy.
  • Bronzato E; Department of Neurosciences, Biomedicine, and Movement Sciences, Policlinico G. B. Rossi, University of Verona, 37134, Verona, Italy.
  • Tamburin S; Department of Neurosciences, Biomedicine, and Movement Sciences, Policlinico G. B. Rossi, University of Verona, 37134, Verona, Italy.
  • Bertolasi L; Department of Neurosciences, Biomedicine, and Movement Sciences, Policlinico G. B. Rossi, University of Verona, 37134, Verona, Italy.
  • Brozzetti L; Department of Neurosciences, Biomedicine, and Movement Sciences, Policlinico G. B. Rossi, University of Verona, 37134, Verona, Italy.
  • Cecchini MP; Department of Neurosciences, Biomedicine, and Movement Sciences, Policlinico G. B. Rossi, University of Verona, 37134, Verona, Italy.
  • Gigli G; Neurology Unit, University of Udine Academic Hospital, 33100, Udine, Italy.
  • Monaco S; Department of Neurosciences, Biomedicine, and Movement Sciences, Policlinico G. B. Rossi, University of Verona, 37134, Verona, Italy.
  • Manganotti P; Neurology Unit, Department of Medicine, Surgery and Health Sciences, Ospedale Cattinara, University of Trieste, 34128, Trieste, Italy.
  • Zanusso G; Department of Neurosciences, Biomedicine, and Movement Sciences, Policlinico G. B. Rossi, University of Verona, 37134, Verona, Italy. gianluigi.zanusso@univr.it.
Transl Neurodegener ; 11(1): 37, 2022 07 28.
Article en En | MEDLINE | ID: mdl-35902902
BACKGROUND: In patients with Parkinson's disease (PD), real-time quaking-induced conversion (RT-QuIC) detection of pathological α-synuclein (α-syn) in olfactory mucosa (OM) is not as accurate as in other α-synucleinopathies. It is unknown whether these variable results might be related to a different distribution of pathological α-syn in OM. Thus, we investigated whether nasal swab (NS) performed in areas with a different coverage by olfactory neuroepithelium, such as agger nasi (AN) and middle turbinate (MT), might affect the detection of pathological α-syn. METHODS: NS was performed in 66 patients with PD and 29 non-PD between September 2018 and April 2021. In 43 patients, cerebrospinal fluid (CSF) was also obtained and all samples were analyzed by RT-QuIC for α-syn. RESULTS: In the first round, 72 OM samples were collected by NS, from AN (NSAN) or from MT (NSMT), and 35 resulted positive for α-syn RT-QuIC, including 27/32 (84%) from AN, 5/11 (45%) from MT, and 3/29 (10%) belonging to the non-PD patients. Furthermore, 23 additional PD patients underwent NS at both AN and MT, and RT-QuIC revealed α-syn positive in 18/23 (78%) NSAN samples and in 10/23 (44%) NSMT samples. Immunocytochemistry of NS preparations showed a higher representation of olfactory neural cells in NSAN compared to NSMT. We also observed α-syn and phospho-α-syn deposits in NS from PD patients but not in controls. Finally, RT-QuIC was positive in 22/24 CSF samples from PD patients (92%) and in 1/19 non-PD. CONCLUSION: In PD patients, RT-QuIC sensitivity is significantly increased (from 45% to 84%) when NS is performed at AN, indicating that α-syn aggregates are preferentially detected in olfactory areas with higher concentration of olfactory neurons. Although RT-QuIC analysis of CSF showed a higher diagnostic accuracy compared to NS, due to the non-invasiveness, NS might be considered as an ancillary procedure for PD diagnosis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedad de Parkinson / Sinucleinopatías Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Transl Neurodegener Año: 2022 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedad de Parkinson / Sinucleinopatías Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Transl Neurodegener Año: 2022 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido