Your browser doesn't support javascript.
loading
Characterization of Xanthomonas arboricola pv. juglandis Bacteriophages against Bacterial Walnut Blight and Field Evaluation.
Retamales, Julio; Núñez, Pablo; Alvarado, Romina; Campan, Erick D M; Otto, Thierry; Segovia, Cristopher; Vasquez, Ignacio; Santander, Javier.
Afiliación
  • Retamales J; Instituto de Ciencias Naturales, Facultad de Medicina Veterinaria y Agronomía, Universidad de las Américas, Viña del Mar 2520000, Chile.
  • Núñez P; Agroadvance SpA, Peñaflor 9750000, Chile.
  • Alvarado R; Agroadvance SpA, Peñaflor 9750000, Chile.
  • Campan EDM; Laboratoire Écologie Fonctionnelle et Environnement, Université de Toulouse, CNRS, 31062 Toulouse, France.
  • Otto T; Laboratoire Écologie Fonctionnelle et Environnement, Université de Toulouse, CNRS, 31062 Toulouse, France.
  • Segovia C; Marine Microbial Pathogenesis and Vaccinology Laboratory, Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, NL A1C 5S7, Canada.
  • Vasquez I; Marine Microbial Pathogenesis and Vaccinology Laboratory, Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, NL A1C 5S7, Canada.
  • Santander J; Marine Microbial Pathogenesis and Vaccinology Laboratory, Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, NL A1C 5S7, Canada.
Viruses ; 14(7)2022 06 24.
Article en En | MEDLINE | ID: mdl-35891361
Xanthomonas arboricola pv. juglandis (hereafter X. juglandis) is the etiological agent of walnut blight, the most important bacterial disease affecting walnut production worldwide. Currently, the disease is treated mainly with copper-derived compounds (e.g., CuSO4) despite the evidence of genetic resistance in these strains. Regarding the effectiveness and sustainability, the use of a bacteriophage appears to be a biocontrol alternative to reduce X. juglandis load and symptomatology of walnut blight. Here, the phages f20-Xaj, f29-Xaj, and f30-Xaj were characterized, and their effectiveness in walnut orchards against walnut blight was determined. These bacteriophages showed a specific lytic infection in X. juglandis strains isolated from Chile and France. Phylogenetic analysis of the complete genome of f20-Xaj and f30-Xaj indicates that these phages belong to the Pradovirus genus. In the field, the cocktail of these bacteriophages showed similar effectivity to CuSO4 in the reduction of incidence and severity in walnut tissue. Moreover, the bacterial load of X. juglandis was significantly reduced in the presence of bacteriophages in contrast to a CuSO4 treatment. These results show that the use of bacteriophages can be an alternative to combat the symptoms of walnut blight caused by X. juglandis.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacteriófagos / Xanthomonas / Juglans Idioma: En Revista: Viruses Año: 2022 Tipo del documento: Article País de afiliación: Chile Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacteriófagos / Xanthomonas / Juglans Idioma: En Revista: Viruses Año: 2022 Tipo del documento: Article País de afiliación: Chile Pais de publicación: Suiza