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Morphological, Molecular, and Biochemical Characterization of a Unique Lentil (Lens culinaris Medik.) Genotype Showing Seed-Coat Color Anomalies Due to Altered Anthocyanin Pathway.
Mishra, Gyan P; Aski, Muraleedhar S; Tontang, Mechiya Tomuilim; Choudhary, Priti; Tripathi, Kuldeep; Singh, Ajeet; Kumar, Ranjeet Ranjan; Thimmegowda, Vinutha; Stobdan, Tsering; Kumar, Atul; Bhardwaj, Rakesh; Praveen, Shelly; Yadava, Devendra Kumar; Kumar, Shiv; Dikshit, Harsh Kumar.
Afiliación
  • Mishra GP; Division of Genetics, Indian Agricultural Research Institute, New Delhi 110012, India.
  • Ankita; Division of Genetics, Indian Agricultural Research Institute, New Delhi 110012, India.
  • Aski MS; Department of Biotechnology, Manav Rachna International Institute of Research and Studies, Faridabad 121004, India.
  • Tontang MT; Division of Genetics, Indian Agricultural Research Institute, New Delhi 110012, India.
  • Choudhary P; Division of Genetics, Indian Agricultural Research Institute, New Delhi 110012, India.
  • Tripathi K; Division of Genetics, Indian Agricultural Research Institute, New Delhi 110012, India.
  • Singh A; Germplasm Evaluation Division, National Bureau of Plant Genetic Resources, New Delhi 110012, India.
  • Kumar RR; Division of Biochemistry, Indian Agricultural Research Institute, New Delhi 110012, India.
  • Thimmegowda V; Division of Biochemistry, Indian Agricultural Research Institute, New Delhi 110012, India.
  • Stobdan T; Division of Biochemistry, Indian Agricultural Research Institute, New Delhi 110012, India.
  • Kumar A; Defence Institute of High Altitude Research, Leh 194101, India.
  • Bhardwaj R; Division of Seed Science and Technology, Indian Agricultural Research Institute, New Delhi 110012, India.
  • Praveen S; Germplasm Evaluation Division, National Bureau of Plant Genetic Resources, New Delhi 110012, India.
  • Yadava DK; Division of Biochemistry, Indian Agricultural Research Institute, New Delhi 110012, India.
  • Kumar S; Crop Science Division, Indian Council of Agricultural Research, New Delhi 110001, India.
  • Dikshit HK; South Asia and China Program, International Center for Agricultural Research in the Dry Areas, New Delhi 110012, India.
Plants (Basel) ; 11(14)2022 Jul 10.
Article en En | MEDLINE | ID: mdl-35890449
This study reports the identification of a unique lentil (Lens culinaris Medik.) genotype L4717-NM, a natural mutant (NM) derived from a variety L4717, producing brown, black, and spotted seed-coat colored seeds in a single plant, generation after generation, in different frequencies. The genetic similarity of L4717 with that of L4717-NM expressing anomalous seed-coat color was established using 54 SSR markers. In addition, various biochemical parameters such as TPC (total phenolic content), TFC (total flavonoid content), DPPH (2,2-diphenyl-1-picrylhydrazyl), FRAP (ferric reducing antioxidant power), H2O2 (peroxide quantification), TCC (total carotenoids content), TAC (total anthocyanin content), and TAA (total ascorbic acid) were also studied in the seeds, sprouts, and seedlings of L4717, brown, black, and spotted seed-coat colored seeds. Stage-specific variations for the key biochemical parameters were recorded, and seedling stage was found the best for many parameters. Moreover, seeds with black seed coat showed better nutraceutical values for most of the studied traits. A highly significant (p ≤ 0.01) and positive correlation was observed between DPPH and TPC, TAA, TFC, etc., whereas, protein content showed a negative correlation with the other studied parameters. The seed coat is maternal tissue and we expect expression of seed-coat color as per the maternal genotype. However, such an anomalous seed-coat expression, which seems to probably be governed by some transposable element in the identified genotype, warrants more detailed studies involving exploitation of the anthocyanin pathway.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Plants (Basel) Año: 2022 Tipo del documento: Article País de afiliación: India Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Plants (Basel) Año: 2022 Tipo del documento: Article País de afiliación: India Pais de publicación: Suiza