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Vascular Remodeling Is a Crucial Event in the Early Phase of Hepatocarcinogenesis in Rodent Models for Liver Tumorigenesis.
Tulessin, Margaret; Sarker, Rim Sabrina Jahan; Griger, Joscha; Leibing, Thomas; Geraud, Cyrill; Weichert, Wilko; Steiger, Katja; Mogler, Carolin.
Afiliación
  • Tulessin M; Institute of Pathology, School of Medicine, Technical University of Munich (TUM), 81675 Munich, Germany.
  • Sarker RSJ; Institute of Pathology, School of Medicine, Technical University of Munich (TUM), 81675 Munich, Germany.
  • Griger J; Comparative Experimental Pathology, School of Medicine, Technical University of Munich (TUM), 81675 Munich, Germany.
  • Leibing T; Institute of Molecular Oncology and Functional Genomics, School of Medicine, Technical University of Munich (TUM), 81675 Munich, Germany.
  • Geraud C; Department of Dermatology, Venereology, and Allergology, University Medical Center and Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany.
  • Weichert W; Department of Dermatology, Venereology, and Allergology, University Medical Center and Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany.
  • Steiger K; Section of Clinical and Molecular Dermatology, University Medical Center and Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany.
  • Mogler C; European Center for Angioscience (ECAS), Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany.
Cells ; 11(14)2022 07 06.
Article en En | MEDLINE | ID: mdl-35883572
The investigation of hepatocarcinogenesis is a major field of interest in oncology research and rodent models are commonly used to unravel the pathophysiology of onset and progression of hepatocellular carcinoma. HCC is a highly vascularized tumor and vascular remodeling is one of the hallmarks of tumor progression. To date, only a few detailed data exist about the vasculature and vascular remodeling in rodent models used for hepatocarcinogenesis. In this study, the vasculature of HCC and the preneoplastic foci of alteration (FCA) of different mouse models with varying genetic backgrounds were comprehensively characterized by using immunohistochemistry (CD31, Collagen IV, αSMA, Desmin and LYVE1) and RNA in situ hybridization (VEGF-A). Computational image analysis was performed to evaluate selected parameters including microvessel density, pericyte coverage, vessel size, intratumoral vessel distribution and architecture using the Aperio ImageScope and Definiens software programs. HCC presented with a significantly lower number of vessels, but larger vessel size and increased coverage, leading to a higher degree of maturation, whereas FCA lesions presented with a higher microvessel density and a higher amount of smaller but more immature vessels. Our results clearly demonstrate that vascular remodeling is present and crucial in early stages of experimental hepatocarcinogenesis. In addition, our detailed characterization provides a strong basis for further angiogenesis studies in these experimental models.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carcinoma Hepatocelular / Neoplasias Hepáticas Límite: Animals Idioma: En Revista: Cells Año: 2022 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carcinoma Hepatocelular / Neoplasias Hepáticas Límite: Animals Idioma: En Revista: Cells Año: 2022 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Suiza