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Fine mapping and candidate gene analysis of gummy stem blight resistance in cucumber stem.
Han, Jianan; Dong, Shaoyun; Shi, Yanxia; Miao, Han; Liu, Xiaoping; Beckles, Diane M; Gu, Xingfang; Zhang, Shengping.
Afiliación
  • Han J; Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
  • Dong S; Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
  • Shi Y; Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
  • Miao H; Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
  • Liu X; Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
  • Beckles DM; Department of Plant Sciences, University of California, Davis, One Shield Avenue, Davis, CA, 95616, USA.
  • Gu X; Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. guxingfang@caas.cn.
  • Zhang S; Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. zhangshengping@caas.cn.
Theor Appl Genet ; 135(9): 3117-3125, 2022 Sep.
Article en En | MEDLINE | ID: mdl-35869997
KEY MESSAGE: Two candidate genes (Csa6G046210 and Csa6G046240) were identified by fine-mapping gsb-s6.2 for gummy stem blight resistance in cucumber stem. Gummy stem blight (GSB) is a serious fungal disease caused by Didymella bryoniae, that affects cucumber yield and quality worldwide. However, no GSB-resistant genes have been identified in cucumber cultivars. In this study, the wild cucumber accession 'PI 183967' was used as a source of resistance to GSB in adult stems. An F2 population was mapped using resistant line 'LM189' and susceptible line 'LM6' derived from a cross between 'PI 183967' and '931'. By developing InDel and SNP markers, the gsb-s6.2 QTL on Chr. 6 was fine-mapped to a 34 kb interval harboring six genes. Gene Expression analysis after inoculation showed that two candidate genes (Csa6G046210 and Csa6G046240) were induced and differentially expressed between the resistant and susceptible parents, and may be involved in disease defense. Sequence alignment showed that Csa6G046210 encodes a multiple myeloma tumor-associated protein, and it harbored two nonsynonymous SNPs and one InDel in the third and the fourth exons, and two InDels in the TATA-box of the basal promoter region. Csa6G046240 encodes a MYB transcription factor with six variants in the AP2/ERF and MYB motifs in the promoter. These two candidate genes lay the foundation for revealing the mechanism of GSB resistance and may be useful for marker-assisted selection in cucumber disease-resistant breeding.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cucumis sativus Tipo de estudio: Prognostic_studies Idioma: En Revista: Theor Appl Genet Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cucumis sativus Tipo de estudio: Prognostic_studies Idioma: En Revista: Theor Appl Genet Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania