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Circulating cancer-associated extracellular vesicles as early detection and recurrence biomarkers for pancreatic cancer.
Yoshioka, Yusuke; Shimomura, Manami; Saito, Keigo; Ishii, Hideshi; Doki, Yuichiro; Eguchi, Hidetoshi; Nakatsura, Tetsuya; Itoi, Takao; Kuroda, Masahiko; Mori, Masaki; Ochiya, Takahiro.
Afiliación
  • Yoshioka Y; Department of Molecular and Cellular Medicine, Institute of Medical Science, Tokyo Medical University, Tokyo, Japan.
  • Shimomura M; Division of Cancer Immunotherapy, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center, Kashiwa, Japan.
  • Saito K; Division of Cancer Immunotherapy, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center, Kashiwa, Japan.
  • Ishii H; Department of Medical Data Science, Center of Medical Innovation and Translational Research, Osaka University Graduate School of Medicine, Suita, Japan.
  • Doki Y; Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita, Japan.
  • Eguchi H; Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita, Japan.
  • Nakatsura T; Division of Cancer Immunotherapy, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center, Kashiwa, Japan.
  • Itoi T; Department of Gastroenterology and Hepatology, Tokyo Medical University, Tokyo, Japan.
  • Kuroda M; Department of Molecular Pathology, Tokyo Medical University, Tokyo, Japan.
  • Mori M; Tokai University School of Medicine, Isehara, Japan.
  • Ochiya T; Department of Molecular and Cellular Medicine, Institute of Medical Science, Tokyo Medical University, Tokyo, Japan.
Cancer Sci ; 113(10): 3498-3509, 2022 Oct.
Article en En | MEDLINE | ID: mdl-35848896
Early detection of pancreatic ductal adenocarcinoma (PDAC) is essential for improving patient survival rates, and noninvasive biomarkers are urgently required to identify patients who are eligible for curative surgery. Here, we examined extracellular vesicles (EVs) from the serum of PDAC patients to determine their ability to detect early-stage disease. EV-associated proteins purified by ultracentrifugation and affinity columns underwent proteomic analysis to identify novel PDAC markers G protein-coupled receptor class C group 5 member C (GPRC5C) and epidermal growth factor receptor pathway substrate 8 (EPS8). To verify the potency of GPRC5C- or EPS8-positive EVs as PDAC biomarkers, we analyzed EVs from PDAC patient blood samples using ultracentrifugation in two different cohorts (a total of 54 PDAC patients, 32 healthy donors, and 22 pancreatitis patients) by immunoblotting. The combination of EV-associated GPRC5C and EPS8 had high accuracy, with area under the curve values of 0.922 and 0.946 for distinguishing early-stage PDAC patients from healthy controls in the two cohorts, respectively, and could detect PDAC patients who were negative for CA19-9. Moreover, we analyzed 30 samples taken at three time points from 10 PDAC patients who underwent surgery: before surgery, after surgery, and recurrence as an early-stage model. These proteins were detected in EVs derived from preoperative and recurrence samples. These results indicated that GPRC5C- or EPS8-positive EVs were biomarkers that have the potential to detect stage I early pancreatic cancer and small recurrent tumors detected by computed tomography.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Pancreáticas / Carcinoma Ductal Pancreático / Vesículas Extracelulares Tipo de estudio: Diagnostic_studies / Prognostic_studies / Risk_factors_studies / Screening_studies Límite: Humans Idioma: En Revista: Cancer Sci Año: 2022 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Pancreáticas / Carcinoma Ductal Pancreático / Vesículas Extracelulares Tipo de estudio: Diagnostic_studies / Prognostic_studies / Risk_factors_studies / Screening_studies Límite: Humans Idioma: En Revista: Cancer Sci Año: 2022 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido