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Design and Initial Characterization of a Small Near-Infrared Fluorescent Calcium Indicator.
Matlashov, Mikhail E; Vera, Jorge; Kasatkina, Ludmila A; Khodakhah, Kamran; Verkhusha, Vladislav V.
Afiliación
  • Matlashov ME; Department of Genetics and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY, United States.
  • Vera J; Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY, United States.
  • Kasatkina LA; Department of Genetics and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY, United States.
  • Khodakhah K; Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY, United States.
  • Verkhusha VV; Department of Genetics and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY, United States.
Front Cell Dev Biol ; 10: 880107, 2022.
Article en En | MEDLINE | ID: mdl-35846350
Near-infrared (NIR) genetically encoded calcium indicators (GECIs) are becoming powerful tools for neuroscience. Because of their spectral characteristics, the use of NIR GECIs helps to avoid signal loss from the absorption by body pigments, light-scattering, and autofluorescence in mammalian tissues. In addition, NIR GECIs do not suffer from cross-excitation artifacts when used with common fluorescent indicators and optogenetics actuators. Although several NIR GECIs have been developed, there is no NIR GECI currently available that would combine the high brightness in cells and photostability with small size and fast response kinetics. Here, we report a small FRET-based NIR fluorescent calcium indicator iGECInano. We characterize iGECInano in vitro, in non-neuronal mammalian cells, and primary mouse neurons. iGECInano demonstrates the improvement in the signal-to-noise ratio and response kinetics compared to other NIR GECIs.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Cell Dev Biol Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Cell Dev Biol Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza