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Characterization and Functional Studies of a Novel Depolymerase Against K19-Type Klebsiella pneumoniae.
Hua, Yunfen; Wu, Yongqin; Guo, Minjie; Ma, Ruijing; Li, Qingchuan; Hu, Zheyuan; Chen, Hongrui; Zhang, Xingyu; Li, Hui; Li, Qingtian; He, Ping.
Afiliación
  • Hua Y; College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, China.
  • Wu Y; Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
  • Guo M; College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, China.
  • Ma R; Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
  • Li Q; Department of Nanoengineering, University of California, San Diego, La Jolla, CA, United States.
  • Hu Z; Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
  • Chen H; NHC Key Laboratory of Parasite and Vector Biology, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, China.
  • Zhang X; Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
  • Li H; Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
  • Li Q; Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
  • He P; Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Front Microbiol ; 13: 878800, 2022.
Article en En | MEDLINE | ID: mdl-35814656
Carbapenem-resistant Klebsiella pneumoniae (CRKP), a pathogen that causes severe nosocomial infections and yields a high mortality rate, poses a serious threat to global public health due to its high antimicrobial resistance. Bacteriophages encode polysaccharide-degrading enzymes referred to as depolymerases that cleave the capsular polysaccharide (CPS), one of the main virulence factors of K. pneumoniae. In this study, we identified and characterized a new capsule depolymerase K19-Dpo41 from K. pneumoniae bacteriophage SH-KP156570. Our characterization of K19-Dpo41 demonstrated that this depolymerase showed specific activities against K19-type K. pneumoniae. K19-Dpo41-mediated treatments promoted the sensitivity of a multidrug-resistant K19-type K. pneumoniae strain to the bactericidal effect of human serum and significantly increased the survival rate of Galleria mellonella infected with K19-type K. pneumoniae. Our results provided strong primary evidence that K19-Dpo41 was not only effective in capsular typing of K19-type K. pneumoniae but promising in terms of developing new alternative therapeutic strategies against K19-type CRKP infections in the future.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Front Microbiol Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Front Microbiol Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza