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Field Evaluation of a Duplex RT-RAA Assay for Rapid Detection of SARS-CoV-2 - Hebei Province, China, January 2021.
Zhang, Ruiqing; Qi, Shunxiang; Li, Jiandong; Liu, Shiyou; Han, Guangyue; Shen, Xinxin; Li, Qi; Xu, Wenbo; Ma, Xuejun.
Afiliación
  • Zhang R; National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.
  • Qi S; Hebei Center for Disease Control and Prevention, Shijiazhuang City, Hebei Province, China.
  • Li J; National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.
  • Liu S; Hebei Center for Disease Control and Prevention, Shijiazhuang City, Hebei Province, China.
  • Han G; Hebei Center for Disease Control and Prevention, Shijiazhuang City, Hebei Province, China.
  • Shen X; National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.
  • Li Q; Hebei Center for Disease Control and Prevention, Shijiazhuang City, Hebei Province, China.
  • Xu W; National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.
  • Ma X; National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.
China CDC Wkly ; 4(23): 504-508, 2022 Jun 10.
Article en En | MEDLINE | ID: mdl-35813263
Introduction: Recently, a local cluster epidemic has occurred in Shijiazhuang City, Hebei Province. Failure to promptly identify patients with fever in rural areas was the major reason for this epidemic. Methods: We presented the field evaluation of a new real-time reverse transcription recombinase-aided amplification (RT-RAA) kit incorporating an endogenous internal control in a single-tube format, completed at the Hebei CDC from January 17, 2021 to January 27, 2021. Results: We evaluated the diagnostic performance of RT-RAA assay using automatic extracted RNA of 808 clinical samples. Compared with reverse transcriptase real-time quantitative PCR (qRT-PCR), RT-RAA kit achieved 92.41% sensitivity, 98.78% specificity and a 96.29% coincidence rate, demonstrating an excellent agreement between the RT-RAA assay and qRT-PCR assay. Furthermore, 58 samples were extracted using a manual extraction method within 5 minutes, but only samples with high nucleic acid concentration (cycle threshold value not higher than 32) could be stably detected. Discussion: The RT-RAA is more suitable to meet the needs of rapid, sensitive, and accurate detection in community-level medical institutions.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies / Guideline Idioma: En Revista: China CDC Wkly Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: China
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies / Guideline Idioma: En Revista: China CDC Wkly Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: China