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Human Blood Platelets Adsorption on Polymeric Materials for Liquid Biopsy.
Potrich, Cristina; Frascella, Francesca; Bertana, Valentina; Barozzi, Mario; Vanzetti, Lia; Piccoli, Federico; Cristallo, Attilio Fabio; Malara, Natalia; Pirri, Candido Fabrizio; Pederzolli, Cecilia; Lunelli, Lorenzo.
Afiliación
  • Potrich C; Fondazione Bruno Kessler, Center for Sensors and Devices, Via Sommarive 18, Povo, 38123 Trento, Italy.
  • Frascella F; Consiglio Nazionale delle Ricerche, Istituto di Biofisica, 38123 Trento, Italy.
  • Bertana V; Department of Applied Science and Technology (DISAT), Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Turin, Italy.
  • Barozzi M; Department of Applied Science and Technology (DISAT), Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Turin, Italy.
  • Vanzetti L; Fondazione Bruno Kessler, Center for Sensors and Devices, Via Sommarive 18, Povo, 38123 Trento, Italy.
  • Piccoli F; Fondazione Bruno Kessler, Center for Sensors and Devices, Via Sommarive 18, Povo, 38123 Trento, Italy.
  • Cristallo AF; Azienda Provinciale per i Servizi Sanitari, S.Chiara Hospital, 38122 Trento, Italy.
  • Malara N; Azienda Provinciale per i Servizi Sanitari, S.Chiara Hospital, 38122 Trento, Italy.
  • Pirri CF; Servizio Trasfusionale Asl 1 Imperiese, 18100 Imperia, Italy.
  • Pederzolli C; Department of Experimental and Clinical Medicine, University of Magna Graecia, 88100 Catanzaro, Italy.
  • Lunelli L; Department of Applied Science and Technology (DISAT), Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Turin, Italy.
Sensors (Basel) ; 22(13)2022 Jun 24.
Article en En | MEDLINE | ID: mdl-35808284
Platelets are emerging as a promising source of blood biomarkers for several pathologies, including cancer. New automated techniques for easier manipulation of platelets in the context of lab-on-a-chips could be of great support for liquid biopsy. Here, several polymeric materials were investigated for their behavior in terms of adhesion and activation of human platelets. Polymeric materials were selected among the most used in microfabrication (PDMS, PMMA and COC) and commercial and home-made resins for 3D printing technology with the aim to identify the most suitable for the realization of microdevices for human platelets isolation and analysis. To visualize adherent platelets and their activation state scanning, electron microscopy was used, while confocal microscopy was used for evaluating platelets' features. In addition, atomic force microscopy was employed to further study platelets adherent to the polymeric materials. Polymers were divided in two main groups: the most prone to platelet adhesion and materials that cause few or no platelets to adhere. Therefore, different polymeric materials could be identified as suitable for the realization of microdevices aimed at capturing human platelets, while other materials could be employed for the fabrication of microdevices or parts of microdevices for the processing of platelets, without loss on surfaces during the process.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plaquetas / Adhesividad Plaquetaria Límite: Humans Idioma: En Revista: Sensors (Basel) Año: 2022 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plaquetas / Adhesividad Plaquetaria Límite: Humans Idioma: En Revista: Sensors (Basel) Año: 2022 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza