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Bactericidal Effect of Ultrasound-Responsive Microbubbles and Sub-inhibitory Gentamicin against Pseudomonas aeruginosa Biofilms on Substrates With Differing Acoustic Impedance.
Plazonic, Filip; LuTheryn, Gareth; Hind, Charlotte; Clifford, Melanie; Gray, Michael; Stride, Eleanor; Glynne-Jones, Peter; Hill, Martyn; Sutton, J Mark; Carugo, Dario.
Afiliación
  • Plazonic F; Faculty of Engineering and Physical Sciences, University of Southampton, Southampton, UK.
  • LuTheryn G; Faculty of Engineering and Physical Sciences, University of Southampton, Southampton, UK; Department of Pharmaceutics, School of Pharmacy, University College London, London, UK; National Biofilms Innovation Centre, University of Southampton, Southampton, UK.
  • Hind C; UK Health Security Agency, Porton Down, Salisbury, Wiltshire, UK.
  • Clifford M; UK Health Security Agency, Porton Down, Salisbury, Wiltshire, UK.
  • Gray M; Institute of Biomedical Engineering, University of Oxford, Oxford, UK.
  • Stride E; Institute of Biomedical Engineering, University of Oxford, Oxford, UK.
  • Glynne-Jones P; Faculty of Engineering and Physical Sciences, University of Southampton, Southampton, UK.
  • Hill M; Faculty of Engineering and Physical Sciences, University of Southampton, Southampton, UK.
  • Sutton JM; UK Health Security Agency, Porton Down, Salisbury, Wiltshire, UK; Institute of Pharmaceutical Science, King's College London, London, UK.
  • Carugo D; Department of Pharmaceutics, School of Pharmacy, University College London, London, UK. Electronic address: D.Carugo@ucl.ac.uk.
Ultrasound Med Biol ; 48(9): 1888-1898, 2022 09.
Article en En | MEDLINE | ID: mdl-35798625
The aim of this research was to explore the interaction between ultrasound-activated microbubbles (MBs) and Pseudomonas aeruginosa biofilms, specifically the effects of MB concentration, ultrasound exposure and substrate properties on bactericidal efficacy. Biofilms were grown using a Centre for Disease Control (CDC) bioreactor on polypropylene or stainless-steel coupons as acoustic analogues for soft and hard tissue, respectively. Biofilms were treated with different concentrations of phospholipid-shelled MBs (107-108 MB/mL), a sub-inhibitory concentration of gentamicin (4 µg/mL) and 1-MHz ultrasound with a continuous or pulsed (100-kHz pulse repetition frequency, 25% duty cycle, 0.5-MPa peak-to-peak pressure) wave. The effect of repeated ultrasound exposure with intervals of either 15- or 60-min was also investigated. With polypropylene coupons, the greatest bactericidal effect was achieved with 2 × 5 min of pulsed ultrasound separated by 60 min and a microbubble concentration of 5 × 107 MBs/mL. A 0.76 log (83%) additional reduction in the number of bacteria was achieved compared with the use of an antibiotic alone. With stainless-steel coupons, a 67% (0.46 log) reduction was obtained under the same exposure conditions, possibly due to enhancement of a standing wave field which inhibited MB penetration in the biofilm. These findings demonstrate the importance of treatment parameter selection in antimicrobial applications of MBs and ultrasound in different tissue environments.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pseudomonas aeruginosa / Microburbujas Idioma: En Revista: Ultrasound Med Biol Año: 2022 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pseudomonas aeruginosa / Microburbujas Idioma: En Revista: Ultrasound Med Biol Año: 2022 Tipo del documento: Article Pais de publicación: Reino Unido