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Homosalate boosts the release of tumour-derived extracellular vesicles with protection against anchorage-loss property.
Grisard, Eleonora; Nevo, Nathalie; Lescure, Aurianne; Doll, Sebastian; Corbé, Maxime; Jouve, Mabel; Lavieu, Gregory; Joliot, Alain; Nery, Elaine Del; Martin-Jaular, Lorena; Théry, Clotilde.
Afiliación
  • Grisard E; Institut Curie, PSL Research University, INSERM U932, Paris, France.
  • Nevo N; Institut Curie, PSL Research University, INSERM U932, Paris, France.
  • Lescure A; Institut Curie, PSL Research University, Translational Research Department, BioPhenics Platform, PICT-IBISA, Paris, France.
  • Doll S; Institute of Metabolism and Cell Death, Helmholtz Zentrum München, Neuherberg, Germany.
  • Corbé M; Institut Curie, PSL Research University, Translational Research Department, BioPhenics Platform, PICT-IBISA, Paris, France.
  • Jouve M; Institut Curie, PSL Research University, CNRS UMR3215, Paris, France.
  • Lavieu G; Institut Curie, PSL Research University, INSERM U932, Paris, France.
  • Joliot A; Université Paris Cité, INSERM U1316, CNRS UMR 7057, Paris, France.
  • Nery ED; Institut Curie, PSL Research University, INSERM U932, Paris, France.
  • Martin-Jaular L; Institut Curie, PSL Research University, Translational Research Department, BioPhenics Platform, PICT-IBISA, Paris, France.
  • Théry C; Institut Curie, PSL Research University, INSERM U932, Paris, France.
J Extracell Vesicles ; 11(7): e12242, 2022 07.
Article en En | MEDLINE | ID: mdl-35790086
Eukaryotic cells, including cancer cells, secrete highly heterogeneous populations of extracellular vesicles (EVs). EVs could have different subcellular origin, composition and functional properties, but tools to distinguish between EV subtypes are scarce. Here, we tagged CD63- or CD9-positive EVs secreted by triple negative breast cancer cells with Nanoluciferase enzyme, to set-up a miniaturized method to quantify secretion of these two EV subtypes directly in the supernatant of cells. We performed a cell-based high-content screening to identify clinically-approved drugs able to affect EV secretion. One of the identified hits is Homosalate, an anti-inflammatory drug found in sunscreens which robustly increased EVs' release. Comparing EVs induced by Homosalate with those induced by Bafilomycin A1, we demonstrate that: (1) the two drugs act on EVs generated in distinct subcellular compartments, and (2) EVs released by Homosalate-, but not by Bafilomycin A1-treated cells enhance resistance to anchorage loss in another recipient epithelial tumour cell line. In conclusion, we identified a new drug modifying EV release and demonstrated that under influence of different drugs, triple negative breast cancer cells release EV subpopulations from different subcellular origins harbouring distinct functional properties.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Mama Triple Negativas / Vesículas Extracelulares Límite: Humans Idioma: En Revista: J Extracell Vesicles Año: 2022 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Mama Triple Negativas / Vesículas Extracelulares Límite: Humans Idioma: En Revista: J Extracell Vesicles Año: 2022 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos