Emerging Themes in CryoEMâSingle Particle Analysis Image Processing.
Chem Rev
; 122(17): 13915-13951, 2022 09 14.
Article
en En
| MEDLINE
| ID: mdl-35785962
Cryo-electron microscopy (CryoEM) has become a vital technique in structural biology. It is an interdisciplinary field that takes advantage of advances in biochemistry, physics, and image processing, among other disciplines. Innovations in these three basic pillars have contributed to the boosting of CryoEM in the past decade. This work reviews the main contributions in image processing to the current reconstruction workflow of single particle analysis (SPA) by CryoEM. Our review emphasizes the time evolution of the algorithms across the different steps of the workflow differentiating between two groups of approaches: analytical methods and deep learning algorithms. We present an analysis of the current state of the art. Finally, we discuss the emerging problems and challenges still to be addressed in the evolution of CryoEM image processing methods in SPA.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Procesamiento de Imagen Asistido por Computador
/
Imagen Individual de Molécula
Idioma:
En
Revista:
Chem Rev
Año:
2022
Tipo del documento:
Article
País de afiliación:
España
Pais de publicación:
Estados Unidos