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Preparation of amphotericin B-loaded hybrid liposomes and the integration of chitin-binding proteins for enhanced antifungal activity.
Santoso, Pugoh; Komada, Takuya; Ishimine, Yugo; Taniguchi, Hiromasa; Minamihata, Kosuke; Goto, Masahiro; Taira, Toki; Kamiya, Noriho.
Afiliación
  • Santoso P; Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Fukuoka 819-0395, Japan.
  • Komada T; Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Fukuoka 819-0395, Japan.
  • Ishimine Y; Department of Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, 1 Senbaru, Nishihara-cho, Okinawa 903-0213, Japan.
  • Taniguchi H; Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Fukuoka 819-0395, Japan.
  • Minamihata K; Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Fukuoka 819-0395, Japan.
  • Goto M; Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Fukuoka 819-0395, Japan.
  • Taira T; Department of Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, 1 Senbaru, Nishihara-cho, Okinawa 903-0213, Japan.
  • Kamiya N; Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Fukuoka 819-0395, Japan; Division of Biotechnology, Center for Future Chemistry, Kyushu University, 744 Mootoka, Nishi-Ku, Fukuoka 819-0395, Japan. Electronic address: kamiya.noriho.367@m.kyushu-u.ac.jp
J Biosci Bioeng ; 134(3): 259-263, 2022 Sep.
Article en En | MEDLINE | ID: mdl-35781189
Amphotericin B (AMB) is a gold standard antifungal drug because of its broad-spectrum activity toward pathogenic yeasts and molds. Because of its low solubility in water and toxicity toward humans, several lipid-based formulations that either increase the aqueous solubility or decrease the side effects have been employed in practical use. In our previous research, we found that the combination of AMB with an artificial palmitoylated chitin-binding domain from Pteris ryukyuensis chitinase (LysM-Pal) resulted in synergistic antifungal action against Trichoderma viride. Herein, we prepared hybrid liposomal formulations by combining a commercially available AMB formulation and liposomes with different surface charges to explore key factors in the antifungal activity. The characterization of AMB-loaded liposomal formulations (AMB-LFs), including particle size distribution and zeta potential, showed that anionic and neutral AMB-LFs could stably encapsulate AMB. The combination of either anionic or neutral AMB-LFs with unmodified LysM decreased the minimum inhibitory concentration of AMB. The combination of neutral AMB-LF with LysM-Pal resulted in a further decrease in the MIC, up to 15-fold compared with that of the neutral AMB-LF alone. Our results demonstrate the potential utility of lipid-based liposomal formulations of AMB combined with lipid-modified proteinaceous binders to tackle fungal infections.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Anfotericina B / Antifúngicos Límite: Humans Idioma: En Revista: J Biosci Bioeng Asunto de la revista: ENGENHARIA BIOMEDICA / MICROBIOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Japón
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Anfotericina B / Antifúngicos Límite: Humans Idioma: En Revista: J Biosci Bioeng Asunto de la revista: ENGENHARIA BIOMEDICA / MICROBIOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Japón