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Nitric oxide detection using catalytic properties of CuCo-PTC metal organic framework.
Wang, Meng; Dong, Hui; Zhang, Yintang; Zhu, Xu; Gu, Mengjiao; Zhu, Qianqian; Miao, Xing; Zhou, Yanli; Xu, Maotian.
Afiliación
  • Wang M; Green Catalysis Center, and College of Chemistry, Zhengzhou University, Zhengzhou, 450001, China.
  • Dong H; Henan Key Laboratory of Biomolecular Recognition and Sensing, College of Chemistry and Chemical Engineering, Henan Joint International Research Laboratory of Chemo/Biosensing and Early Diagnosis of Major Diseases, Shangqiu Normal University, Shangqiu, 476000, China.
  • Zhang Y; Green Catalysis Center, and College of Chemistry, Zhengzhou University, Zhengzhou, 450001, China. huidong607607@126.com.
  • Zhu X; Henan Key Laboratory of Biomolecular Recognition and Sensing, College of Chemistry and Chemical Engineering, Henan Joint International Research Laboratory of Chemo/Biosensing and Early Diagnosis of Major Diseases, Shangqiu Normal University, Shangqiu, 476000, China. huidong607607@126.com.
  • Gu M; Green Catalysis Center, and College of Chemistry, Zhengzhou University, Zhengzhou, 450001, China. ahhh_cs@163.com.
  • Zhu Q; Henan Key Laboratory of Biomolecular Recognition and Sensing, College of Chemistry and Chemical Engineering, Henan Joint International Research Laboratory of Chemo/Biosensing and Early Diagnosis of Major Diseases, Shangqiu Normal University, Shangqiu, 476000, China. ahhh_cs@163.com.
  • Miao X; Henan Key Laboratory of Biomolecular Recognition and Sensing, College of Chemistry and Chemical Engineering, Henan Joint International Research Laboratory of Chemo/Biosensing and Early Diagnosis of Major Diseases, Shangqiu Normal University, Shangqiu, 476000, China.
  • Zhou Y; Henan Key Laboratory of Biomolecular Recognition and Sensing, College of Chemistry and Chemical Engineering, Henan Joint International Research Laboratory of Chemo/Biosensing and Early Diagnosis of Major Diseases, Shangqiu Normal University, Shangqiu, 476000, China.
  • Xu M; Henan Key Laboratory of Biomolecular Recognition and Sensing, College of Chemistry and Chemical Engineering, Henan Joint International Research Laboratory of Chemo/Biosensing and Early Diagnosis of Major Diseases, Shangqiu Normal University, Shangqiu, 476000, China.
Mikrochim Acta ; 189(7): 263, 2022 07 01.
Article en En | MEDLINE | ID: mdl-35776229
As a vital gaseous signal molecule involved in various physiological and pathological processes, nitric oxide (NO) has attracted extensive attention in the last few decades. In this work, a copper and cobalt element-doped, biphenyl-(3, 4', 5)-tricarboxylic acid (H3PTC)-synthesized metal organic framework (CuCo-PTC MOF) composite with catalytic ability was synthesized by solvothermal method. The material can catalyse the oxidation of o-phenylenediamine (OPD) groups by hydrogen peroxide (H2O2) to form fluorophores (OPDox) with yellow fluorescence emission and greatly improves its reaction rate. In the presence of NO, OPD will react with NO to produce N-(2-hydrazinophenyl) methylamine, and the group will not react with H2O2. Therefore, the concentration of NO can be measured indirectly by comparing the changes of fluorescence intensity in the presence and absence of NO. As the concentration of NO changes, the change of solution colour (from bright yellow to colourless) can also be observed under a 365-nm UV lamp. Furthermore, the method represents high selectivity for NO and shows a fast (within 5 min) and specific fluorescence response toward NO with a linear range from 0.25 to 2.0 µM; the strategy has a limit of detection (LOD) of  0.15 µM. More importantly, the probe was successfully used to detect NO in cell lysate. The recovery was between 98.5 and 103.6%, and the relative standard deviation was between 0.4 and 1.8%. The endogenous NO in cells was successfully detected under the stimulation of L-arginine, which proved the possibility of the probe in real-time and rapid sensing in actual samples and cells. The results indicate that this sensing strategy has the potential to detect NO in the internal environment. Schematic of fluorescence detection of NO.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estructuras Metalorgánicas Tipo de estudio: Diagnostic_studies Idioma: En Revista: Mikrochim Acta Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Austria
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estructuras Metalorgánicas Tipo de estudio: Diagnostic_studies Idioma: En Revista: Mikrochim Acta Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Austria