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Natural Killer Cell Expansion and Cytotoxicity Differ Depending on the Culture Medium Used.
Koh, Seung Kwon; Park, Jeehun; Kim, Seong-Eun; Lim, Yuree; Phan, Minh-Trang Thi; Kim, Jinho; Hwang, Ilwoong; Ahn, Yong-Oon; Shin, Sue; Doh, Junsang; Cho, Duck.
Afiliación
  • Koh SK; Department of Health Sciences and Technology, Samsung Advanced Institute for Health Sciences and Technology (SAIHST), Sungkyunkwan University, Seoul, Korea.
  • Park J; Research Institute of Advanced Materials (RIAM), Seoul National University, Seoul, Korea.
  • Kim SE; Department of Mechanical Engineering, Pohang University of Science and Technology, Pohang, Korea.
  • Lim Y; Department of Biopharmaceutical Convergence, Sungkyunkwan University (SKKU), Suwon, Korea.
  • Phan MT; Cell and Gene Therapy Institute (CGTI), Samsung Medical Center, Seoul, Korea.
  • Kim J; Department of Health Sciences and Technology, Samsung Advanced Institute for Health Sciences and Technology (SAIHST), Sungkyunkwan University, Seoul, Korea.
  • Hwang I; Department of Emergency Medicine, Soonchunhyang University Gumi Hospital, Gumi, Korea.
  • Ahn YO; Department of Pediatrics, Columbia University Irving Medical Center, New York, NY, USA.
  • Shin S; Department of Laboratory Medicine, Seoul National University, Seoul, Korea.
  • Doh J; Department of Laboratory Medicine, Seoul Metropolitan Government-Seoul National University (SMG-SNU) Boramae Hospital, Seoul, Korea.
  • Cho D; Research Institute of Advanced Materials (RIAM), Seoul National University, Seoul, Korea.
Ann Lab Med ; 42(6): 638-649, 2022 Nov 01.
Article en En | MEDLINE | ID: mdl-35765872
Background: Adoptive cell therapy using umbilical cord blood (UCB)-derived allogeneic natural killer (NK) cells has shown encouraging results. However, because of the insufficient availability of NK cells and limited UCB volume, more effective culture methods are required. NK cell expansion and functionality are largely affected by the culture medium. While human serum is a major affecting component in culture media, the way it regulates NK cell functionality remains elusive. We elucidated the effects of different culture media and human serum supplementation on UCB NK cell expansion and functionality. Methods: UCB NK cells were cultured under stimulation with K562-OX40L-mbIL-18/21 feeder cells and IL-2 and IL-15 in serum-containing and serum-free culture media. The effects of the culture media and human serum supplementation on NK cell expansion and cytotoxicity were evaluated by analyzing the expansion rate, activating and inhibitory receptor levels, and the cytotoxicity of the UCB NK cells. Results: The optimal medium for NK cell expansion was Dulbecco's modified Eagle's medium/Ham's F12 with supplements and that for cytotoxicity was AIM V supplemented with Immune Cell Serum Replacement. Shifting media is an advantageous strategy for obtaining several highly functional UCB NK cells. Live cell imaging and killing time measurement revealed that human serum enhanced NK cell proliferation but delayed target recognition, resulting in reduced cytotoxicity. Conclusions: Culture medium supplementation with human serum strongly affects UCB NK cell expansion and functionality. Thus, culture media should be carefully selected to ensure both NK cell quantity and quality for adoptive cell therapy.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Asesinas Naturales Límite: Humans Idioma: En Revista: Ann Lab Med Año: 2022 Tipo del documento: Article Pais de publicación: Corea del Sur
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Asesinas Naturales Límite: Humans Idioma: En Revista: Ann Lab Med Año: 2022 Tipo del documento: Article Pais de publicación: Corea del Sur