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Urease and α-Chymotrypsin Inhibitory Activities and Molecular Docking Studies of Alkaloids Isolated from Medicinal Plant Isatis minima Bunge.
Fozia, Fozia; Ahmad, Ijaz; Haq, Zia Ul; Wadood, Abdul; Khan, Hidayat Ullah; Ahmed, Mushtaq; Jamila, Nargis; Ullah, Riaz; Alotaibi, Amal; Sultan, Mujeeb A.
Afiliación
  • Fozia F; Boichemistry Department, Khyber Medical University Institute of Medical Sciences, Kohat, Khyber Pakhtunkhwa, Pakistan.
  • Ahmad I; Department of Chemistry, Kohat University of Science & Technology, Kohat, Khyber Pakhtunkhwa, Pakistan.
  • Haq ZU; Institute of Public Health & Social Sciences, Khyber Medical University, Peshawar, Khyber Pakhtunkhwa, Pakistan.
  • Wadood A; Department of Biochemistry, Abdul Wali Khan University Mardan, Khyber Pakhtunkhwa, Pakistan.
  • Khan HU; Department of Chemistry, University of Science & Technology, Bannu, Khyber Pakhtunkhwa, Pakistan.
  • Ahmed M; Department of Biotechnology, University of Science & Technology, Bannu, Khyber Pakhtunkhwa, Pakistan.
  • Jamila N; Department of Chemistry, Shaheed Benazir Bhutto Women University, Peshawar, Khyber Pakhtunkhwa, Pakistan.
  • Ullah R; Department of Pharmacognosy (MAPPRC, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
  • Alotaibi A; Department of Basic Science, College of Medicine, Princess Nourah Bint Abdulrahman University, P. O. Box 84428, Riyadh 11671, Saudi Arabia.
  • Sultan MA; Department of Pharmacy, Faculty of Medical Sciences, Aljanad University for Science and Technology, Taiz, Yemen.
Evid Based Complement Alternat Med ; 2022: 1904874, 2022.
Article en En | MEDLINE | ID: mdl-35754682
Phytochemical studies on the alkaloids fraction of the entire plant of Isatis minima Bunge resulted in the alkaloids 1-4 isolation, which were first time isolated from this species. The 1D and 2D NMR spectroscopic data were used to identify their structures, and there was satisfactory compatibility of the data compared to those which were previously published. In the examined compounds 1-4, Isaindigotidione (3) and Isaindigotone (4) were shown as an effective urease inhibitor in such a concentration-dependent way against Jack bean and Bacillus pasteurii urease, with IC50 values 29.03 ± 0.04, 20.04 ± 0.09 and 34.03 ± 0.07, 26.13 ± 0.08 µM, respectively. Compounds 3 and 4 were likewise shown to be an effective inhibitor against α-chymotrypsin, exhibiting IC50 values 16.09 ± 0.07 and 22.01 ± 0.06 µM, correspondingly. The program MOE-Dock was used to perform a molecular docking analysis to confirm probable binding modes of the active complexes of the isolated compounds 1-4 and the crystal structure of urease and α-chymotrypsin enzymes. Compound 3 was the most active, having the highest docking scores against Bacillus pasteurii urease, α-chymotrypsin, and Jack bean (-8.6876), (-7.6647), and (-13.1927) µM, respectively. All four alkaloids (1-4) showed significant urease and protease inhibitory potential and further these activities were confirmed with the help of molecular docking study.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Evid Based Complement Alternat Med Año: 2022 Tipo del documento: Article País de afiliación: Pakistán Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Evid Based Complement Alternat Med Año: 2022 Tipo del documento: Article País de afiliación: Pakistán Pais de publicación: Estados Unidos