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Kinetic model supported improved and optimized submerged production strategy of cellulase enzyme from newspaper waste biomass.
Dey, Pinaki; Chakrabortty, Sankha; Haldar, Dibyajyoti; Sowmya, A; Rangarajan, Vivek; Ruiz, Héctor A.
Afiliación
  • Dey P; Department of Biotechnology, Karunya Institute of Technology and Sciences, Coimbatore, Tamil Nadu, 641114, India. saspinaki@gmail.com.
  • Chakrabortty S; School of Chemical Engineering, Kalinga Institute of Industrial Technology, Bhubaneswar, 751024, India.
  • Haldar D; Department of Biotechnology, Karunya Institute of Technology and Sciences, Coimbatore, Tamil Nadu, 641114, India.
  • Sowmya A; Department of Biotechnology, Karunya Institute of Technology and Sciences, Coimbatore, Tamil Nadu, 641114, India.
  • Rangarajan V; Department of Chemical Engineering, BITS Pilani, KK Birla Goa Campus, Sancoale, Goa, 403726, India.
  • Ruiz HA; Biorefinery Group, Food Research Department, Faculty of Chemistry Sciences, Autonomous University of Coahuila, 25280, Saltillo, Coahuila, Mexico.
Bioprocess Biosyst Eng ; 45(8): 1281-1295, 2022 Aug.
Article en En | MEDLINE | ID: mdl-35750867
A systematic evaluation of microorganism's potential towards biosynthesis of cellulases from inexpensive lignocellulosic feedstock through appropriate kinetic modelling facilitates understanding, optimization and designing of an effective industrial cellulase enzyme production process. The present study aims to optimize a submerged fungal cultivation strategy for cellulase production from abundantly available newspaper wastes (NPW). A combined pretreatment strategy consisting diluted, 1% (v v-1) H2SO4 followed by 2% (w v-1) NaOH treatment was highly effective to convert newspaper waste to an effective cellulose-enriched inducer for the production of cellulase. In addition, the composition of the most influential nutrient components like peptone and lactose was optimized with the help of response surface methodology for enhanced cellulase production with maximum activity levels. Maximum cellulase production of 8.64 g L-1 with 7.82 FPU mL-1 total activity levels was achieved from optimized composition of pretreated NPW 3.29% (w v-1), lactose 2.94% (w v-1) and peptone 1.53% (w v-1). To analyse intrinsic inhibition effect of the substrate concentration on cellulase production, modified Luedeking-Piret model simulated experiments were further conducted with 1.5% (w/v), 3.29% (w/v) and 4% (w/v) NPW concentrations. The developed kinetic model perfectly captured the trends of biomass production, substrate consumption and adsorption characteristic of cellulase enzyme on its activity during production. The rate constant for cellulase synthesis was evaluated to be increased to 0.040 IU g-1 h -1 at 3.29% (w v-1) of NPW concentration; however, it was further reduced to 0.024 IU g-1 h -1 at higher NPW concentration of 4% (w v-1).
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Celulasa / Celulasas Tipo de estudio: Prognostic_studies Idioma: En Revista: Bioprocess Biosyst Eng Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2022 Tipo del documento: Article País de afiliación: India Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Celulasa / Celulasas Tipo de estudio: Prognostic_studies Idioma: En Revista: Bioprocess Biosyst Eng Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2022 Tipo del documento: Article País de afiliación: India Pais de publicación: Alemania