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PhageScore-based analysis of Acinetobacter baumannii infecting phages antibiotic interaction in liquid medium.
Grygorcewicz, Bartlomiej; Roszak, Marta; Rakoczy, Rafal; Augustyniak, Adrian; Konopacki, Maciej; Jablonska, Joanna; Serwin, Natalia; Cecerska-Heryc, Elzbieta; Kordas, Marian; Galant, Katarzyna; Dolegowska, Barbara.
Afiliación
  • Grygorcewicz B; Chair of Microbiology, Department of Laboratory Medicine, Immunology and Laboratory Medicine, Pomeranian Medical University in Szczecin, Powstanców Wielkopolskich 72, 70-111, Szczecin, Poland. bartlomiej.grygorcewicz@pum.edu.pl.
  • Roszak M; Department of Chemical and Process Engineering, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology in Szczecin, Piastów Avenue 42, 71-065, Szczecin, Poland. bartlomiej.grygorcewicz@pum.edu.pl.
  • Rakoczy R; Chair of Microbiology, Department of Laboratory Medicine, Immunology and Laboratory Medicine, Pomeranian Medical University in Szczecin, Powstanców Wielkopolskich 72, 70-111, Szczecin, Poland.
  • Augustyniak A; Department of Chemical and Process Engineering, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology in Szczecin, Piastów Avenue 42, 71-065, Szczecin, Poland.
  • Konopacki M; Department of Chemical and Process Engineering, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology in Szczecin, Piastów Avenue 42, 71-065, Szczecin, Poland.
  • Jablonska J; Chair of Microbiology, Department of Laboratory Medicine, Immunology and Laboratory Medicine, Pomeranian Medical University in Szczecin, Powstanców Wielkopolskich 72, 70-111, Szczecin, Poland.
  • Serwin N; Department of Chemical and Process Engineering, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology in Szczecin, Piastów Avenue 42, 71-065, Szczecin, Poland.
  • Cecerska-Heryc E; Department of Chemical and Process Engineering, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology in Szczecin, Piastów Avenue 42, 71-065, Szczecin, Poland.
  • Kordas M; Chair of Microbiology, Department of Laboratory Medicine, Immunology and Laboratory Medicine, Pomeranian Medical University in Szczecin, Powstanców Wielkopolskich 72, 70-111, Szczecin, Poland.
  • Galant K; Chair of Microbiology, Department of Laboratory Medicine, Immunology and Laboratory Medicine, Pomeranian Medical University in Szczecin, Powstanców Wielkopolskich 72, 70-111, Szczecin, Poland.
  • Dolegowska B; Department of Chemical and Process Engineering, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology in Szczecin, Piastów Avenue 42, 71-065, Szczecin, Poland.
Arch Microbiol ; 204(7): 421, 2022 Jun 24.
Article en En | MEDLINE | ID: mdl-35748948
The growing interest in bacteriophages and antibiotics' combined use poses new challenges regarding this phenomenon's accurate description. This study aimed to apply the PhageScore methodology to assess the phage-antibiotic combination activity in liquid bacterial culture. For this purpose, previously described Acinetobacter infecting phages vB_AbaP_AGC01, Aba-1, and Aba-4 and antibiotics (gentamicin, ciprofloxacin, meropenem, norfloxacin, and fosfomycin) were used to obtain a lysis curve of bacteriophages under antibiotic pressure. The experimental data were analyzed using the Fractional Inhibitory Concentration Index (FICI) and PhageScore methodology. The results obtained by this method clearly show differences between phage lytic activity after antibiotic addition. Thus, we present the potential use of the PhageScore method as a tool for characterizing the phage antibiotic synergy in liquid culture. Further, the optimization of the PhageScore for this purpose can help compare antibiotics and their outcome on bacteriophage lytic activity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacteriófagos / Acinetobacter baumannii Idioma: En Revista: Arch Microbiol Año: 2022 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacteriófagos / Acinetobacter baumannii Idioma: En Revista: Arch Microbiol Año: 2022 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Alemania