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Whole-Transcriptome Analysis of Non-Coding RNA Alteration in Porcine Alveolar Macrophage Exposed to Aflatoxin B1.
Chao, Huhe; Ma, Haohai; Sun, Jiadong; Yuan, Shuai; Dong, Peiyu; Zhao, Aihong; Li, Lan; Shen, Wei; Zhang, Xifeng.
Afiliación
  • Chao H; College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China.
  • Ma H; Central Laboratory, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100023, China.
  • Sun J; College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China.
  • Yuan S; Key Laboratory of Animal Reproduction and Biotechnology in Universities of Shandong, College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China.
  • Dong P; School of Medicine, Henan Polytechnic University, Jiaozuo 454000, China.
  • Zhao A; College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China.
  • Li L; Qingdao Academy of Agricultural Science, Qingdao 266100, China.
  • Shen W; Key Laboratory of Animal Reproduction and Biotechnology in Universities of Shandong, College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China.
  • Zhang X; Key Laboratory of Animal Reproduction and Biotechnology in Universities of Shandong, College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China.
Toxins (Basel) ; 14(6)2022 05 27.
Article en En | MEDLINE | ID: mdl-35737034
Aflatoxin B1 (AFB1) is a type of mycotoxin produced by the fungi Aspergillus flavus and Aspergillus parasiticus and is commonly found in cereals, oils and foodstuffs. In order to understand the toxic effects of AFB1 exposure on Porcine alveolar macrophages (3D4/2 cell), the 3D4/2 cells were exposed to 40 µg/mL AFB1 for 24 h in vitro, and several methods were used for analysis. Edu and TUNEL analysis showed that the proliferation of 3D4/2 cells was significantly inhibited and the apoptosis of 3D4/2 cells was significantly induced after AFB1 exposure compared with that of the control group. Whole-transcriptome analysis was performed to reveal the non-coding RNA alteration in 3D4/2 cells after AFB1 exposure. It was found that the expression of cell-cycle-related and apoptosis-related genes was altered after AFB1 exposure, and lncRNAs and miRNAs were also significantly different among the experimental groups. In particular, AFB1 exposure affected the expression of lncRNAs associated with cellular senescence signaling pathways, such as MSTRG.24315 and MSTRG.80767, as well as related genes, Cxcl8 and Gadd45g. In addition, AFB1 exposure affected the expression of miRNAs associated with immune-related genes, such as miR-181a, miR-331-3p and miR-342, as well as immune-related genes Nfkb1 and Rras2. Moreover, the regulation networks between mRNA-miRNAs and mRNA-lncRNAs were confirmed by the results of RT-qPCR and immunofluorescence. In conclusion, our results here demonstrate that AFB1 exposure impaired proliferation of 3D4/2 cells via the non-coding RNA-mediated pathway.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: MicroARNs / ARN Largo no Codificante Límite: Animals Idioma: En Revista: Toxins (Basel) Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
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Imprimir
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: MicroARNs / ARN Largo no Codificante Límite: Animals Idioma: En Revista: Toxins (Basel) Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza